A swine influenza virus (SIV) vaccine-challenge pig model was used to study the potential of a conserved matrix 2 (M2) protein vaccine alone or in combination with an inactivated H1N1-vaccine to protect against H1N1 and H1N2 viruses. The H1N1-vaccine and heterologous H1N2-challenge virus model has previously been shown to prolong fever and increase SIV-associated pneumonic lesions. The M2 vaccine in combination with the H1N1-vaccine reduced the H1N2 induced fever but not virus shedding. The M2 vaccine alone reduced respiratory signs and pneumonic lesions to levels similar to the negative control pigs following H1N2 infection. This study found that the M2 protein has potential as a vaccine for SIV-associated disease prevention. However, development of an immune response towards the major envelope HA protein was required to reduce SIV shedding.
Bibliographical noteFunding Information:
The authors thank Dr. Cornelia Schroeder (Abteilung Virologie, Institut für Mikrobiologie und Hygiene, Universität des Saarlandes, Homburg, Germany) for her guidance in rM2 protein purification. We also would like to thank Nancy Upchurch and the students in the Thacker lab for their assistance in this project. This work was supported by a grant from Iowa Livestock Health Advisory Council .
Copyright 2010 Elsevier B.V., All rights reserved.
- M2 vaccine
- Swine influenza virus