Swine dysentery disease mechanism: Brachyspira hampsonii impairs the colonic immune and epithelial repair responses to induce lesions

Matheus O. Costa, John C.S. Harding

Research output: Contribution to journalArticlepeer-review

Abstract

Swine dysentery (SD) is a global, production-limiting disease of pigs in commercial farms. It is associated with infection by Brachyspira hyodysenteriae and B. hampsonii, and characterized by mucohaemorrhagic diarrhea and colitis, SD prevention, treatment or control relies heavily on antimicrobials as no commercial vaccines are available. This is linked to our poor understanding of the disease pathogenesis. Our goal was to characterize the host-pathogen interactions during the early stage of infection. We employed dual RNA-seq to profile mRNA and miRNA following 1-h incubation of colonic explants with a pathogenic or a non-pathogenic B. hampsonii strain. Our results suggest that the pathogenic strain more efficiently interfered with the host's ability to activate and build a humoral response (through IL-4/CCR6/KLHL6 interactions), epithelial wound repair mechanisms (associated with LSECtin impairment of macrophages), induced mitochondrial dysfunction (linked to MDR1), and loss of microbiome homeostasis. The pathogenic strain also up-regulated the expression of stress-associated genes, when compared to the non-pathogenic strain. These results shed a light on the pathophysiological mechanisms that lead to SD and will contribute to the development of novel disease control tools.

Original languageEnglish (US)
Article number104470
JournalMicrobial Pathogenesis
Volume148
DOIs
StatePublished - Nov 2020

Bibliographical note

Funding Information:
The authors would like to thanks Roman Nosach and Ana Paula Poeta Silva for technical assistance.

Keywords

  • Brachyspira
  • Colitis
  • Diarrhea
  • Immunosupression
  • Mechanisms
  • Mucohaemorrhagic
  • Pathogenesis
  • Swine dysentery
  • Transcriptome

PubMed: MeSH publication types

  • Journal Article

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