Swelling of Collagen-Hyaluronic Acid Co-Gels: An In Vitro Residual Stress Model

Victor K. Lai, David S. Nedrelow, Spencer P. Lake, Bumjun Kim, Emily M. Weiss, Robert T. Tranquillo, Victor H. Barocas

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Tissue-equivalents (TEs), simple model tissues with tunable properties, have been used to explore many features of biological soft tissues. Absent in most formulations however, is the residual stress that arises due to interactions among components with different unloaded levels of stress, which has an important functional role in many biological tissues. To create a pre-stressed model system, co-gels were fabricated from a combination of hyaluronic acid (HA) and reconstituted Type-I collagen (Col). When placed in solutions of varying osmolarity, HA-Col co-gels swell as the HA imbibes water, which in turn stretches (and stresses) the collagen network. In this way, co-gels with residual stress (i.e., collagen fibers in tension and HA in compression) were fabricated. When the three gel types tested here were immersed in hypotonic solutions, pure HA gels swelled the most, followed by HA-Col co-gels; no swelling was observed in pure collagen gels. The greatest swelling rates and swelling ratios occurred in the lowest salt concentration solutions. Tension on the collagen component of HA-Col co-gels was calculated from a stress balance and increased nonlinearly as swelling increased. The swelling experiment results were in good agreement with the stress predicted by a fibril network + non-fibrillar interstitial matrix computational model.

Original languageEnglish (US)
Pages (from-to)2984-2993
Number of pages10
JournalAnnals of Biomedical Engineering
Issue number10
StatePublished - Oct 1 2016

Bibliographical note

Funding Information:
This work was supported by the National Institute of Health (RO1 EB005813), and by a resources grant from the Minnesota Supercomputing Institute. There are no conflicts of interest.


  • Biomechanics
  • Donnan
  • Fiber
  • Multiscale
  • Network
  • Osmotic
  • Pre-stress
  • Tissue

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