SV40 associated miRNAs are not detectable in mesotheliomas

G. V. Gee, M. L. Stanifer, B. C. Christensen, W. J. Atwood, D. Ugolini, S. Bonassi, M. B. Resnick, H. H. Nelson, C. J. Marsit, K. T. Kelsey

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Background:Simian virus-40 (SV40) is a DNA tumour virus that was introduced into the human population with contaminated poliovirus vaccine, and its role in mesothelioma is widely debated. PCR based testing has been called into question, as false positives can be because of cross-reactivity with related viruses, or to laboratory contamination. The Institute of Medicine has recommended the development of more sensitive and specific tests to resolve this controversy.Methods:We have characterized highly sensitive RT-PCR based assays that are specific for SV40-encoded microRNAs (miRNAs), as an alternative to current testing methods.Results:Using this sensitive and specific detection method, we were unable to identify SV40 miRNA expression in human malignant pleural mesothelioma (MM) samples.Conclusion:Our work indicates that SV40 miRNAs are not likely to contribute to mesothelioma tumourogenesis, but highlights the value of this approach when compared with the relatively unspecific current testing methods.

Original languageEnglish (US)
Pages (from-to)885-888
Number of pages4
JournalBritish Journal of Cancer
Volume103
Issue number6
DOIs
StatePublished - Sep 7 2010

Keywords

  • SV40
  • mesothelioma
  • microRNA

Fingerprint Dive into the research topics of 'SV40 associated miRNAs are not detectable in mesotheliomas'. Together they form a unique fingerprint.

Cite this