Sustained neuronal activation raises oxidative metabolism to a new steady-state level: Evidence from 1H NMR spectroscopy in the human visual cortex

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Abstract

To date, functional 1H NMR spectroscopy has been utilized to report the time courses of few metabolites, primarily lactate. Benefiting from the sensitivity offered by ultra-high magnetic field (7 T), the concentrations of 17 metabolites were measured in the human visual cortex during two paradigms of visual stimulation lasting 5.3 and 10.6 mins. Significant concentration changes of approximately 0.2 μmol/g were observed for several metabolites: lactate increased by 23%±5% (P<0.0005), glutamate increased by 3%±1% (P<0.01), whereas aspartate decreased by 15%±6% (P<0.05). Glucose concentration also manifested a tendency to decrease during activation periods. The lactate concentration reached the new steady-state level within the first minute of activation and came back to baseline only after the stimulus ended. The changes of the concentration of metabolites implied a rise in oxidative metabolism to a new steady-state level during activation and indicated that amino-acid homeostasis is affected by physiological stimulation, likely because of an increased flux through the malate-aspartate shuttle.

Original languageEnglish (US)
Pages (from-to)1055-1063
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume27
Issue number5
DOIs
StatePublished - May 16 2007

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Visual Cortex
Lactic Acid
Magnetic Resonance Spectroscopy
Aspartic Acid
Aminoacylation
Photic Stimulation
Magnetic Fields
Glutamic Acid
Homeostasis
Glucose
Proton Magnetic Resonance Spectroscopy

Keywords

  • BOLD
  • Brain activation
  • Functional metabolism
  • H NMR spectroscopy
  • In vivo
  • Lactate
  • Malate-aspartate shuttle

Cite this

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title = "Sustained neuronal activation raises oxidative metabolism to a new steady-state level: Evidence from 1H NMR spectroscopy in the human visual cortex",
abstract = "To date, functional 1H NMR spectroscopy has been utilized to report the time courses of few metabolites, primarily lactate. Benefiting from the sensitivity offered by ultra-high magnetic field (7 T), the concentrations of 17 metabolites were measured in the human visual cortex during two paradigms of visual stimulation lasting 5.3 and 10.6 mins. Significant concentration changes of approximately 0.2 μmol/g were observed for several metabolites: lactate increased by 23{\%}±5{\%} (P<0.0005), glutamate increased by 3{\%}±1{\%} (P<0.01), whereas aspartate decreased by 15{\%}±6{\%} (P<0.05). Glucose concentration also manifested a tendency to decrease during activation periods. The lactate concentration reached the new steady-state level within the first minute of activation and came back to baseline only after the stimulus ended. The changes of the concentration of metabolites implied a rise in oxidative metabolism to a new steady-state level during activation and indicated that amino-acid homeostasis is affected by physiological stimulation, likely because of an increased flux through the malate-aspartate shuttle.",
keywords = "BOLD, Brain activation, Functional metabolism, H NMR spectroscopy, In vivo, Lactate, Malate-aspartate shuttle",
author = "Silvia Mangia and Ivan Tk{\'a}č and Rolf Gruetter and {Van De Moortele}, {Pierre Francois} and Bruno Maraviglia and K{\^a}mil Uǧurbil",
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T1 - Sustained neuronal activation raises oxidative metabolism to a new steady-state level

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AU - Mangia, Silvia

AU - Tkáč, Ivan

AU - Gruetter, Rolf

AU - Van De Moortele, Pierre Francois

AU - Maraviglia, Bruno

AU - Uǧurbil, Kâmil

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N2 - To date, functional 1H NMR spectroscopy has been utilized to report the time courses of few metabolites, primarily lactate. Benefiting from the sensitivity offered by ultra-high magnetic field (7 T), the concentrations of 17 metabolites were measured in the human visual cortex during two paradigms of visual stimulation lasting 5.3 and 10.6 mins. Significant concentration changes of approximately 0.2 μmol/g were observed for several metabolites: lactate increased by 23%±5% (P<0.0005), glutamate increased by 3%±1% (P<0.01), whereas aspartate decreased by 15%±6% (P<0.05). Glucose concentration also manifested a tendency to decrease during activation periods. The lactate concentration reached the new steady-state level within the first minute of activation and came back to baseline only after the stimulus ended. The changes of the concentration of metabolites implied a rise in oxidative metabolism to a new steady-state level during activation and indicated that amino-acid homeostasis is affected by physiological stimulation, likely because of an increased flux through the malate-aspartate shuttle.

AB - To date, functional 1H NMR spectroscopy has been utilized to report the time courses of few metabolites, primarily lactate. Benefiting from the sensitivity offered by ultra-high magnetic field (7 T), the concentrations of 17 metabolites were measured in the human visual cortex during two paradigms of visual stimulation lasting 5.3 and 10.6 mins. Significant concentration changes of approximately 0.2 μmol/g were observed for several metabolites: lactate increased by 23%±5% (P<0.0005), glutamate increased by 3%±1% (P<0.01), whereas aspartate decreased by 15%±6% (P<0.05). Glucose concentration also manifested a tendency to decrease during activation periods. The lactate concentration reached the new steady-state level within the first minute of activation and came back to baseline only after the stimulus ended. The changes of the concentration of metabolites implied a rise in oxidative metabolism to a new steady-state level during activation and indicated that amino-acid homeostasis is affected by physiological stimulation, likely because of an increased flux through the malate-aspartate shuttle.

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KW - In vivo

KW - Lactate

KW - Malate-aspartate shuttle

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