Susceptibility of Nocardia asteroides to 45 antimicrobial agents in vitro.

M. C. Bach, L. D. Sabath, M. Finland

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A method for preparing uniformly dispersed cultures of Nocardia asteroides for use in tests for susceptibility to antimicrobial agents is described. The minimal inhibiting concentration (MIC) of 45 agents for cultures thus prepared was determined with the use of a replica-inoculating apparatus. Minocycline at a concentration of 3.1 mug or less/ml inhibited 90% of the strains tested, and all were inhibited by 6.3 mug/ml. An erythromycin concentration of 0.8 mug or less/ml inhibited 40% of the strains, but the MIC for most of the others was > 100 mug/ml. The other agents were generally less active. Chemically related analogues varied in activity to different degrees. Also, the MIC of each antibiotic against different strains generally varied over a wide range. Sulfonamides and trimethoprim were not active against most strains in the method used. The size of the inoculum markedly affected the MIC of sulfonamides and had variable effects on other agents. Marked synergy of erythromycin with ampicillin was demonstrated for nearly all strains tested.
Original languageUndefined/Unknown
Pages (from-to)1-8
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume3
Issue number1
StatePublished - 1973

Keywords

  • Anti-Bacterial Agents/pd [Pharmacology]
  • Drug Synergism
  • Microbial Sensitivity Tests
  • Nocardia asteroides/de [Drug Effects]

Cite this

Susceptibility of Nocardia asteroides to 45 antimicrobial agents in vitro. / Bach, M. C.; Sabath, L. D.; Finland, M.

In: Antimicrobial Agents and Chemotherapy, Vol. 3, No. 1, 1973, p. 1-8.

Research output: Contribution to journalArticle

@article{1ef7ac55b1224a7fac1334937de4c767,
title = "Susceptibility of Nocardia asteroides to 45 antimicrobial agents in vitro.",
abstract = "A method for preparing uniformly dispersed cultures of Nocardia asteroides for use in tests for susceptibility to antimicrobial agents is described. The minimal inhibiting concentration (MIC) of 45 agents for cultures thus prepared was determined with the use of a replica-inoculating apparatus. Minocycline at a concentration of 3.1 mug or less/ml inhibited 90{\%} of the strains tested, and all were inhibited by 6.3 mug/ml. An erythromycin concentration of 0.8 mug or less/ml inhibited 40{\%} of the strains, but the MIC for most of the others was > 100 mug/ml. The other agents were generally less active. Chemically related analogues varied in activity to different degrees. Also, the MIC of each antibiotic against different strains generally varied over a wide range. Sulfonamides and trimethoprim were not active against most strains in the method used. The size of the inoculum markedly affected the MIC of sulfonamides and had variable effects on other agents. Marked synergy of erythromycin with ampicillin was demonstrated for nearly all strains tested.",
keywords = "Anti-Bacterial Agents/pd [Pharmacology], Drug Synergism, Microbial Sensitivity Tests, Nocardia asteroides/de [Drug Effects]",
author = "Bach, {M. C.} and Sabath, {L. D.} and M. Finland",
year = "1973",
language = "Undefined/Unknown",
volume = "3",
pages = "1--8",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Susceptibility of Nocardia asteroides to 45 antimicrobial agents in vitro.

AU - Bach, M. C.

AU - Sabath, L. D.

AU - Finland, M.

PY - 1973

Y1 - 1973

N2 - A method for preparing uniformly dispersed cultures of Nocardia asteroides for use in tests for susceptibility to antimicrobial agents is described. The minimal inhibiting concentration (MIC) of 45 agents for cultures thus prepared was determined with the use of a replica-inoculating apparatus. Minocycline at a concentration of 3.1 mug or less/ml inhibited 90% of the strains tested, and all were inhibited by 6.3 mug/ml. An erythromycin concentration of 0.8 mug or less/ml inhibited 40% of the strains, but the MIC for most of the others was > 100 mug/ml. The other agents were generally less active. Chemically related analogues varied in activity to different degrees. Also, the MIC of each antibiotic against different strains generally varied over a wide range. Sulfonamides and trimethoprim were not active against most strains in the method used. The size of the inoculum markedly affected the MIC of sulfonamides and had variable effects on other agents. Marked synergy of erythromycin with ampicillin was demonstrated for nearly all strains tested.

AB - A method for preparing uniformly dispersed cultures of Nocardia asteroides for use in tests for susceptibility to antimicrobial agents is described. The minimal inhibiting concentration (MIC) of 45 agents for cultures thus prepared was determined with the use of a replica-inoculating apparatus. Minocycline at a concentration of 3.1 mug or less/ml inhibited 90% of the strains tested, and all were inhibited by 6.3 mug/ml. An erythromycin concentration of 0.8 mug or less/ml inhibited 40% of the strains, but the MIC for most of the others was > 100 mug/ml. The other agents were generally less active. Chemically related analogues varied in activity to different degrees. Also, the MIC of each antibiotic against different strains generally varied over a wide range. Sulfonamides and trimethoprim were not active against most strains in the method used. The size of the inoculum markedly affected the MIC of sulfonamides and had variable effects on other agents. Marked synergy of erythromycin with ampicillin was demonstrated for nearly all strains tested.

KW - Anti-Bacterial Agents/pd [Pharmacology]

KW - Drug Synergism

KW - Microbial Sensitivity Tests

KW - Nocardia asteroides/de [Drug Effects]

M3 - Article

VL - 3

SP - 1

EP - 8

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 1

ER -