Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins

Liping Zhang, Fangneng Huang, B. Rogers Leonard, Mao Chen, Thomas Clark, Yu Cheng Zhu, David S. Wangila, Fei Yang, Ying Niu

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Sugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F2 families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) of resistance to Cry1Ab as shown in the known Cry1Ab-resistant strain, while resistance levels were relatively lower for other strains (116- to 129-fold). All the six strains were highly cross-resistant to Cry1Aa (71- to 292-fold) and Cry1Ac (30- to 248-fold), but only with a low level to Cry1F (<7-fold). Larval growth of all six strains was also inhibited on Bt-treated diet, but, except for Cry1F, the growth inhibition of the six strains was considerably less than that of the Cry1Ab-susceptible larvae. The results provide clear evidence that the observed resistance to Cry1Ab maize in the six strains is a result of resistance to the Cry1Ab protein in the plants. The low level of cross-resistance between Cry1A and Cry1F suggests that pyramiding these two types of Bt proteins into a plant could be a good strategy for managing D. saccharalis.

Original languageEnglish (US)
Pages (from-to)267-272
Number of pages6
JournalJournal of Invertebrate Pathology
Volume112
Issue number3
DOIs
StatePublished - Mar 2013
Externally publishedYes

Bibliographical note

Funding Information:
This article is published with the approval of the Director of the Louisiana Agricultural Experiment Station as manuscript No. 2012-234-7574. This project represents work supported by the Louisiana Soybean and Feed Grain Promotion Board, USDA-AFRI South Regional IPM program, Monsanto Company, and USDA NC-205.

Keywords

  • Bacillus thuringiensis
  • Bt maize
  • Cross-resistance
  • Diatraea saccharalis
  • Resistance management

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