Susceptibility of broiler chicks to infection by avian pneumovirus of turkey origin

H. J. Shin, B. McComb, A. Back, D. P. Shaw, D. A. Halvorson, K. V. Nagaraja

Research output: Contribution to journalArticle

  • 14 Citations

Abstract

In this paper we present the results of studies on the infectivity of an isolate of avian pneumovirus (APV) from turkeys to broiler chickens. Two-week-old broiler chicks free of antibodies to APV were exposed either by oculonasal or oral route with a cell cultured APV of turkey origin. Chickens from both APV-inoculated groups exhibited clinical signs that included coughing, sneezing, nasal discharge, and watery eyes during 2-8 days postinoculation. Tissue samples from birds in the APV-inoculated group were positive for APV by polymerase chain reaction (PCR) up to 9 days postinoculation. Samples of blood from both oculonasally and orally infected chickens were positive for APV. Intestinal samples from chickens infected with APV orally were positive for the presence of APV on PCR up to 9 days postinoculation. APV was reisolated from samples taken from chickens in both groups inoculated orally and oculonasally. Sera from birds exposed by the oculonasal or by the oral route showed the presence of APV-specific antibodies.

Original languageEnglish (US)
Pages (from-to)797-802
Number of pages6
JournalAvian Diseases
Volume44
Issue number4
StatePublished - 2000

Fingerprint

Metapneumovirus
Infection
Avian metapneumovirus
Chickens
chickens
sampling
broiler chickens
Birds
Polymerase Chain Reaction
Antibodies
polymerase chain reaction
antibodies
birds
Sneezing
Nose
Cultured Cells
Serum
blood serum
cultured cells
pathogenicity

Keywords

  • APV
  • Broiler chickens
  • ELISA
  • RT-PCR
  • Turkey
  • Virus isolation

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

Cite this

Susceptibility of broiler chicks to infection by avian pneumovirus of turkey origin. / Shin, H. J.; McComb, B.; Back, A.; Shaw, D. P.; Halvorson, D. A.; Nagaraja, K. V.

In: Avian Diseases, Vol. 44, No. 4, 2000, p. 797-802.

Research output: Contribution to journalArticle

Shin, H. J.; McComb, B.; Back, A.; Shaw, D. P.; Halvorson, D. A.; Nagaraja, K. V. / Susceptibility of broiler chicks to infection by avian pneumovirus of turkey origin.

In: Avian Diseases, Vol. 44, No. 4, 2000, p. 797-802.

Research output: Contribution to journalArticle

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