Survey of US wastewater for carbapenem-resistant Enterobacteriaceae

Jill Hoelle, James R Johnson, Brian D Johnston, Brian Kinkle, Laura Boczek, Hodon Ryu, Sam Hayes

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A survey for antibiotic-resistant (AR) Escherichia coli in wastewater was undertaken by collecting samples from primary clarifiers and secondary effluents from seven geographically dispersed US wastewater treatment plants (WWTPs). Samples were collected at each WWTP in cool and summer months and cultured using selective media. The resulting isolates were characterized for resistance to imipenem, ciprofloxacin, cefotaxime, and ceftazidime, presence of carbapenemase and extended-spectrum beta-lactamase (ESBL) genes, and phylogroups and sequence types (STs). In total, 322 AR E. coli isolates were identified, of which 65 were imipenem-resistant. Of the 65 carbapenem-resistant E. coli (CREC) isolates, 62% were positive for more than one and 31% were positive for two or more of carbapenemase and ESBL genes targeted. The most commonly detected carbapenemase gene was blaVIM (n ¼ 36), followed by blaKPC (n ¼ 2). A widespread dispersal of carbapenem-resistant STs and other clinically significant AR STs observed in the present study suggested the plausible release of these strains into the environment. The occurrence of CREC in wastewater is a potential concern because this matrix may serve as a reservoir for gene exchange and thereby increase the risk of AR bacteria (including CR) being disseminated into the environment and thence back to humans.

Original languageEnglish (US)
Pages (from-to)219-226
Number of pages8
JournalJournal of Water and Health
Volume17
Issue number2
DOIs
StatePublished - Apr 1 2019

Fingerprint

Carbapenems
Enterobacteriaceae
Waste Water
antibiotics
Escherichia coli
Anti-Bacterial Agents
wastewater
Imipenem
gene
beta-Lactamases
Genes
Ceftazidime
Cefotaxime
Ciprofloxacin
effluent
Bacteria
bacterium
matrix
Surveys and Questionnaires
summer

Keywords

  • Carbapenem-resistant E. coli
  • Carbapenemases
  • ESBL
  • Wastewater

PubMed: MeSH publication types

  • Journal Article

Cite this

Survey of US wastewater for carbapenem-resistant Enterobacteriaceae. / Hoelle, Jill; Johnson, James R; Johnston, Brian D; Kinkle, Brian; Boczek, Laura; Ryu, Hodon; Hayes, Sam.

In: Journal of Water and Health, Vol. 17, No. 2, 01.04.2019, p. 219-226.

Research output: Contribution to journalArticle

Hoelle, Jill ; Johnson, James R ; Johnston, Brian D ; Kinkle, Brian ; Boczek, Laura ; Ryu, Hodon ; Hayes, Sam. / Survey of US wastewater for carbapenem-resistant Enterobacteriaceae. In: Journal of Water and Health. 2019 ; Vol. 17, No. 2. pp. 219-226.
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