Surgical induction, histological evaluation, and MRI identification of cartilage necrosis in the distal femur in goats to model early lesions of osteochondrosis

F. Tóth, M. J. Nissi, L. Wang, J. M. Ellermann, C. S. Carlson

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Objective: Identify and interrupt the vascular supply to portions of the distal femoral articular-epiphyseal cartilage complex (AECC) in goat kids to induce cartilage necrosis, characteristic of early lesions of osteochondrosis (OC); then utilize magnetic resonance imaging (MRI) to identify necrotic areas of cartilage. Design: Distal femora were perfused and cleared in goat kids of various ages to visualize the vascular supply to the distal femoral AECC. Vessels located on the axial aspect of the medial femoral condyle (MFC) and on the abaxial side of the lateral trochlear ridge were transected in eight 4- to 5-day-old goats to induce cartilage necrosis. Goats were euthanized 1, 2, 3, 4, 5, 6, 9, and 10 weeks post operatively and operated stifles were harvested. Adiabatic T1ρ relaxation time maps of the harvested distal femora were generated using a 9.4T MR scanner, after which samples were evaluated histologically. Results: Interruption of the vascular supply to the MFC caused lesions of cartilage necrosis in 6/8 goat kids that were demonstrated histologically. Adiabatic T1ρ relaxation time mapping identified these areas of cartilage necrosis in 5/6 cases. No significant findings were detected after transection of perichondrial vessels supplying the lateral trochlear ridge. Conclusions: Cartilage necrosis, characteristic of early OC, can be induced by interrupting the vascular supply to the distal femoral AECC in goat kids. The ability of high field MRI to identify these areas of cartilage necrosis in the AECC using the adiabatic T1ρ sequence suggests that this technique may be useful in the future for the early diagnosis of OC.

Original languageEnglish (US)
Pages (from-to)300-307
Number of pages8
JournalOsteoarthritis and Cartilage
Volume23
Issue number2
DOIs
StatePublished - Feb 1 2015

Bibliographical note

Funding Information:
Support from NIH grants T32OD010993 , K18OD010468 , P41 EB015894 , R21AR065385 , W.M. KECK Foundation , and University of Minnesota College of Veterinary Medicine Comparative Medicine Faculty Grant were used during the completion of the study.

Publisher Copyright:
© 2014 Osteoarthritis Research Society International.

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

Keywords

  • Adiabatic T1ρ
  • Cartilage necrosis
  • Goat
  • MRI
  • Osteochondrosis

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