Surface modification for protein and DNA immobilization onto GMR biosensor

Wei Wang; Yi Wang; Liang Tu; Todd Klein; Yinglong Feng; Jianping Wang

doi:10.1109/TMAG.2012.2224327

Surface modification for protein and DNA immobilization onto GMR biosensor

Wei Wang
, Yi Wang
, Liang Tu
, Todd Klein
, Yinglong Feng
, Jianping Wang

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Giant magnetoresistance (GMR) biosensor with 20 nm SiO₂ on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.

Original language	English (US)
Article number	6392433
Pages (from-to)	296-299
Number of pages	4
Journal	IEEE Transactions on Magnetics
Volume	49
Issue number	1
DOIs	https://doi.org/10.1109/TMAG.2012.2224327
State	Published - 2013

Keywords

Deoxyribonucleic acid (DNA)
giant magnetoresistance (GMR) biosensor
immobilization
protein
surface modification

Publisher link

10.1109/TMAG.2012.2224327

Find It

Find It at U of M Libraries

Cite this

@article{83689bff05864197bcbce69ce2c9838c,

title = "Surface modification for protein and DNA immobilization onto GMR biosensor",

abstract = "Giant magnetoresistance (GMR) biosensor with 20 nm SiO2 on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.",

keywords = "Deoxyribonucleic acid (DNA), giant magnetoresistance (GMR) biosensor, immobilization, protein, surface modification",

author = "Wei Wang and Yi Wang and Liang Tu and Todd Klein and Yinglong Feng and Jianping Wang",

year = "2013",

doi = "10.1109/TMAG.2012.2224327",

language = "English (US)",

volume = "49",

pages = "296--299",

journal = "IEEE Transactions on Magnetics",

issn = "0018-9464",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

number = "1",

}

TY - JOUR

T1 - Surface modification for protein and DNA immobilization onto GMR biosensor

AU - Wang, Wei

AU - Wang, Yi

AU - Tu, Liang

AU - Klein, Todd

AU - Feng, Yinglong

AU - Wang, Jianping

PY - 2013

Y1 - 2013

N2 - Giant magnetoresistance (GMR) biosensor with 20 nm SiO2 on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.

AB - Giant magnetoresistance (GMR) biosensor with 20 nm SiO2 on surface was successfully modified by 3-aminopropyltriethoxy silane (APTES) and glutaraldehyde (Glu). The resultant functionalized surface with terminal aldehyde groups was able to efficiently capture Interleukin-6 (IL-6) antibody and amine modified DNA (deoxyribonucleic acid) oligonucleotide. The immobilized IL-6 antibody could bind to IL-6 antigen, and fluorescence sandwich assay was demonstrated. The immobilized DNA could also hybridize with complementary DNA oligonucleotide. Streptavidin labeled magnetic nanoparticles with a diameter of 30 nm were both successfully bound to IL-6 antibody and DNA immobilized GMR biosensors after their respective sandwich binding and complementary hybridization. This APTES-Glu modification method could be also applicable to other surface for protein and DNA microarrays.

KW - Deoxyribonucleic acid (DNA)

KW - giant magnetoresistance (GMR) biosensor

KW - immobilization

KW - protein

KW - surface modification

UR - https://www.scopus.com/pages/publications/84871822291

UR - https://www.scopus.com/pages/publications/84871822291#tab=citedBy

U2 - 10.1109/TMAG.2012.2224327

DO - 10.1109/TMAG.2012.2224327

M3 - Article

AN - SCOPUS:84871822291

SN - 0018-9464

VL - 49

SP - 296

EP - 299

JO - IEEE Transactions on Magnetics

JF - IEEE Transactions on Magnetics

IS - 1

M1 - 6392433

ER -

Surface modification for protein and DNA immobilization onto GMR biosensor

Abstract

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this