The feasibility of employing the surface coil probe technique for the non-invasive study of ocular tissue metabolism by phosphorus-31 nuclear magnetic resonance spectroscopy (31P NMR) in enucleated bovine, rabbit, human and rat globes is demonstrated. An assessment of individual phosphorus-metabolite contributions from ocular tissues, including the cornea, lens and iris, to the overall 31P NMR spectrum (NMR spectral acquisition parameters optimized for the lens region of the globe) was accomplished through the combination of surgical ablation and difference spectroscopy. The NMR measurements also provided tissue pH values for the lens and cornea. The strengths and limitations of the surface coil NMR method, which is particularly appropriate for in vivo metabolic studies of ocular tissues such as the lens, are discussed.
Bibliographical noteFunding Information:
for his assistance in providing human tissue and Dr Patricia N. Farnsworth for incisive comments. This research was supported by grants from the American Diabetes Association, the Estate of M. J. Davidson, the W. M. Keck Foundation and the National Institutes of Health (EY 04033). P. Campbell was supported by a Fight for Sight (New York City) Fellowship; G. Acosta received Fellowship support from the U.S. Department of Education Graduate and Professional Opportunity Program.
- bovine globe
- cornea pH
- human globe
- lens pH
- nuclear magnetic resonance
- nuclear magnetic resonance spectroscopy
- phosphorus-31 nuclear magnetic resonance spectroscopy
- rabbit globe
- rat globe
- surface coil