Surface coil phosphorus-31 nuclear magnetic resonance studies of the intact eye

Thomas Schleich; Gerald B. Matson; James A. Willis; Galo Acosta; Christian Serdahl; Patrick Campbell; Michael Garwood

doi:10.1016/0014-4835(85)90148-4

Surface coil phosphorus-31 nuclear magnetic resonance studies of the intact eye

Thomas Schleich, Gerald B. Matson, James A. Willis, Galo Acosta, Christian Serdahl, Patrick Campbell, Michael Garwood

Radiology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The feasibility of employing the surface coil probe technique for the non-invasive study of ocular tissue metabolism by phosphorus-31 nuclear magnetic resonance spectroscopy (³¹P NMR) in enucleated bovine, rabbit, human and rat globes is demonstrated. An assessment of individual phosphorus-metabolite contributions from ocular tissues, including the cornea, lens and iris, to the overall ³¹P NMR spectrum (NMR spectral acquisition parameters optimized for the lens region of the globe) was accomplished through the combination of surgical ablation and difference spectroscopy. The NMR measurements also provided tissue pH values for the lens and cornea. The strengths and limitations of the surface coil NMR method, which is particularly appropriate for in vivo metabolic studies of ocular tissues such as the lens, are discussed.

Original language	English (US)
Pages (from-to)	343-355
Number of pages	13
Journal	Experimental Eye Research
Volume	40
Issue number	3
DOIs	https://doi.org/10.1016/0014-4835(85)90148-4
State	Published - Mar 1985

Bibliographical note

Funding Information:
for his assistance in providing human tissue and Dr Patricia N. Farnsworth for incisive comments. This research was supported by grants from the American Diabetes Association, the Estate of M. J. Davidson, the W. M. Keck Foundation and the National Institutes of Health (EY 04033). P. Campbell was supported by a Fight for Sight (New York City) Fellowship; G. Acosta received Fellowship support from the U.S. Department of Education Graduate and Professional Opportunity Program.

Keywords

bovine globe
cornea
cornea pH
human globe
lens
lens pH
nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
phosphorus-31 nuclear magnetic resonance spectroscopy
rabbit globe
rat globe
surface coil

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title = "Surface coil phosphorus-31 nuclear magnetic resonance studies of the intact eye",

abstract = "The feasibility of employing the surface coil probe technique for the non-invasive study of ocular tissue metabolism by phosphorus-31 nuclear magnetic resonance spectroscopy (31P NMR) in enucleated bovine, rabbit, human and rat globes is demonstrated. An assessment of individual phosphorus-metabolite contributions from ocular tissues, including the cornea, lens and iris, to the overall 31P NMR spectrum (NMR spectral acquisition parameters optimized for the lens region of the globe) was accomplished through the combination of surgical ablation and difference spectroscopy. The NMR measurements also provided tissue pH values for the lens and cornea. The strengths and limitations of the surface coil NMR method, which is particularly appropriate for in vivo metabolic studies of ocular tissues such as the lens, are discussed.",

keywords = "bovine globe, cornea, cornea pH, human globe, lens, lens pH, nuclear magnetic resonance, nuclear magnetic resonance spectroscopy, phosphorus-31 nuclear magnetic resonance spectroscopy, rabbit globe, rat globe, surface coil",

author = "Thomas Schleich and Matson, {Gerald B.} and Willis, {James A.} and Galo Acosta and Christian Serdahl and Patrick Campbell and Michael Garwood",

note = "Funding Information: for his assistance in providing human tissue and Dr Patricia N. Farnsworth for incisive comments. This research was supported by grants from the American Diabetes Association, the Estate of M. J. Davidson, the W. M. Keck Foundation and the National Institutes of Health (EY 04033). P. Campbell was supported by a Fight for Sight (New York City) Fellowship; G. Acosta received Fellowship support from the U.S. Department of Education Graduate and Professional Opportunity Program.",

year = "1985",

month = mar,

doi = "10.1016/0014-4835(85)90148-4",

language = "English (US)",

volume = "40",

pages = "343--355",

journal = "Experimental Eye Research",

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T1 - Surface coil phosphorus-31 nuclear magnetic resonance studies of the intact eye

AU - Schleich, Thomas

AU - Matson, Gerald B.

AU - Willis, James A.

AU - Acosta, Galo

AU - Serdahl, Christian

AU - Campbell, Patrick

AU - Garwood, Michael

N1 - Funding Information: for his assistance in providing human tissue and Dr Patricia N. Farnsworth for incisive comments. This research was supported by grants from the American Diabetes Association, the Estate of M. J. Davidson, the W. M. Keck Foundation and the National Institutes of Health (EY 04033). P. Campbell was supported by a Fight for Sight (New York City) Fellowship; G. Acosta received Fellowship support from the U.S. Department of Education Graduate and Professional Opportunity Program.

PY - 1985/3

Y1 - 1985/3

N2 - The feasibility of employing the surface coil probe technique for the non-invasive study of ocular tissue metabolism by phosphorus-31 nuclear magnetic resonance spectroscopy (31P NMR) in enucleated bovine, rabbit, human and rat globes is demonstrated. An assessment of individual phosphorus-metabolite contributions from ocular tissues, including the cornea, lens and iris, to the overall 31P NMR spectrum (NMR spectral acquisition parameters optimized for the lens region of the globe) was accomplished through the combination of surgical ablation and difference spectroscopy. The NMR measurements also provided tissue pH values for the lens and cornea. The strengths and limitations of the surface coil NMR method, which is particularly appropriate for in vivo metabolic studies of ocular tissues such as the lens, are discussed.

AB - The feasibility of employing the surface coil probe technique for the non-invasive study of ocular tissue metabolism by phosphorus-31 nuclear magnetic resonance spectroscopy (31P NMR) in enucleated bovine, rabbit, human and rat globes is demonstrated. An assessment of individual phosphorus-metabolite contributions from ocular tissues, including the cornea, lens and iris, to the overall 31P NMR spectrum (NMR spectral acquisition parameters optimized for the lens region of the globe) was accomplished through the combination of surgical ablation and difference spectroscopy. The NMR measurements also provided tissue pH values for the lens and cornea. The strengths and limitations of the surface coil NMR method, which is particularly appropriate for in vivo metabolic studies of ocular tissues such as the lens, are discussed.

KW - bovine globe

KW - cornea

KW - cornea pH

KW - human globe

KW - lens

KW - lens pH

KW - nuclear magnetic resonance

KW - nuclear magnetic resonance spectroscopy

KW - phosphorus-31 nuclear magnetic resonance spectroscopy

KW - rabbit globe

KW - rat globe

KW - surface coil

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AN - SCOPUS:0022318912

SN - 0014-4835

VL - 40

SP - 343

EP - 355

JO - Experimental Eye Research

JF - Experimental Eye Research

IS - 3

ER -

Surface coil phosphorus-31 nuclear magnetic resonance studies of the intact eye

Abstract

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