Abstract
Supraphysiological temperatures are generated by radiofrequency and microwave probes used for the treatment of prostate cancer (Montrosi et al., 1992) and benign prostatic hyperplasia (Larson et al., 1996). A quantitative understanding of the cellular mechanisms of tissue destruction due to these supraphysiological temperatures(> 40°C) is necessary for optimal application of clinical therapeutic protocols on the prostate and other tissue systems. A multitude of biophysical and biochemical events take place at the cellular level due to thermal stress (Cravalho et al., 1992). Some of the events include hyperpermeability of the membrane, denaturation of proteins, changes in cytoskeleton, alteration of intracellular ionic concentration and nuclear degradation. This study quantifies membrane injury by measuring the dynamics of vital dye leakage (Calcein) and Propidium Iodide (PI) uptake in the AT-1 Dunning rat prostate tumor cell line. Membrane injury (using these two dyes) is compared to the clonogenicity of these cells after comparable thermal insult. An Arrhenius damage model has been constructed for each of these assays based on a damage parameter to obtain the activation Energy (E) and frequency factor (A), which may prove useful in obtaining insight into the mechanisms of damage associated with thermal injury.
| Original language | English (US) |
|---|---|
| Title of host publication | Advances in Heat and Mass Transfer in Biotechnology |
| Publisher | American Society of Mechanical Engineers (ASME) |
| Pages | 77-78 |
| Number of pages | 2 |
| Volume | 40 |
| ISBN (Electronic) | 9780791815984 |
| DOIs | |
| State | Published - Dec 1 1998 |
| Event | ASME 1998 International Mechanical Engineering Congress and Exposition, IMECE 1998 - Anaheim, United States Duration: Nov 15 1998 → Nov 20 1998 |
Publication series
| Name | American Society of Mechanical Engineers, Bioengineering Division (Publication) BED |
|---|---|
| ISSN (Print) | 1071-6947 |
Conference
| Conference | ASME 1998 International Mechanical Engineering Congress and Exposition, IMECE 1998 |
|---|---|
| Country/Territory | United States |
| City | Anaheim |
| Period | 11/15/98 → 11/20/98 |
Bibliographical note
Funding Information:ACKNOWLEDGMENTS The authors would like to thank Ken Roberts and David Swanlund for suggestions and help during the course of this study, and Dr. C. W. Song’s lab. for technical assistance. This research was funded in part by a land-grant from the University of Minnesota to John C. Bischof.
Publisher Copyright:
© 1998 American Society of Mechanical Engineers (ASME). All rights reserved.
