Suppression of the interleukin-2 gene expression by aflatoxin B1 is mediated through the down-regulation of the NF-AT and AP-1 transcription factors

Seung Hyun Han, Young J Jeon, Sung Su Yea, Kyu Hwan Yang

Research output: Contribution to journalArticle

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Abstract

The effect of aflatoxin B1 (AFB1) on the interleukin-2 (IL-2) gene expression was investigated in thymocytes of B6C3F1 mice, Jurkat E6-1 human T-cell leukemia, and EL4.IL-2 murine thymoma. AFB1 inhibited the phorbol-12-myristate-13-acetate/ionomycin (PMA/Io)-induced IL-2 mRNA expression in the murine thymocytes and Jurkat E6-1 cells as determined by qualitative RT-PCR, while no effect was observed in the EL4.IL-2 cells. Electrophoretic mobility shift assay indicated that AFB1 treatment showed an inhibition of the NF-AT and AP-1 DNA binding in PMA/Io-stimulated thymocytes and Jurkat E6-1 cells. No effect was observed on the Oct and NF-κB DNA binding. Employing a reporter gene expression system with p(NF-AT)3-CAT and p(AP-1)3-CAT, treatment with AFB1 to the transfected Jurkat E6-1 cells also showed an inhibition of the PMA/Io-induced NF-AT/CAT and AP-1/CAT activities. These results suggest that suppression of the IL-2 gene expression by AFB1 is mediated through the down-regulation of the NF-AT and AP-1 activation. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalToxicology Letters
Volume108
Issue number1
DOIs
StatePublished - Jul 30 1999

Fingerprint

Aflatoxin B1
Transcription Factor AP-1
Gene expression
Interleukin-2
Down-Regulation
Ionomycin
Gene Expression
Thymocytes
Acetates
T-Cell Leukemia
Electrophoretic mobility
Thymoma
T-cells
DNA
Electrophoretic Mobility Shift Assay
Reporter Genes
Assays
Chemical activation
Polymerase Chain Reaction
Messenger RNA

Keywords

  • AP-1
  • Aflatoxin B
  • Immunosuppression
  • Interleukin-2
  • NF-AT

Cite this

Suppression of the interleukin-2 gene expression by aflatoxin B1 is mediated through the down-regulation of the NF-AT and AP-1 transcription factors. / Han, Seung Hyun; Jeon, Young J; Yea, Sung Su; Yang, Kyu Hwan.

In: Toxicology Letters, Vol. 108, No. 1, 30.07.1999, p. 1-10.

Research output: Contribution to journalArticle

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