Abstract
The influence of ageing on the expression of two superoxide dismutase (SOD) isozymes was examined in three different skeletal muscle fiber types of young (Y, 8 mo) and old (O, 25 mo) rats. Total SOD activity was increased with age in the gastrocnemius (Gas, type II(mix)) and superficial vastus lateralis (SVL, type IIb) but unchanged in the soleus (Sol, type I). The increased SOD activity in SVL was due to increased cytosolic SOD (CuZn SOD), whereas both mitochondrial (Mn SOD) and CuZn SOD activities were increased in Gas. In Sol, Mn SOD activity was significantly increased in aged rats. Mn SOD mRNA level was significantly decreased with age in all three muscles examined, while Mn SOD protein content was not altered. Ageing did not affect CuZn SOD mRNA abundance in any of the muscles, but significantly increased CuZn SOD protein content in aged Gas and Sol. Binding of two redox-sensitive transcription factors, nuclear factor-κB (NFκB) and activator protein-1 (AP-1) was significantly decreased with age in all three muscle types. These results indicate that increased SOD activity in aged skeletal muscle is not associated with higher levels of gene transcription. Increases in Mn SOD activity seen in aged Gas and Sol are the result of post-translational modification of the enzyme, whereas increases in CuZn SOD activity during ageing may be due to both translational and post-translational control. Copyright (C) 2000 Elsevier Science Ireland Ltd.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 33-45 |
| Number of pages | 13 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 116 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 10 2000 |
| Externally published | Yes |
Bibliographical note
Funding Information:This study was supported in part by the American Federation for Ageing Research and by the Vilas Trust Fund of the University of Wisconsin–Madison. John Hollander is a recipient of a Glenn/AFAR scholarship. The cDNA probes for Mn SOD and CuZn SOD were kind gifts from Dr. Ye-Shih Ho, Wayne State University, Detroit, Michigan. The cDNA probe for 18S was a kind gift from Dr. Karyn Esser, University of Illinois, Chicago, Illinois. The Mn SOD and CuZn SOD ELISA antibodies and purified standards were kind gifts from Dr. Keiichiro Suzuki and Dr. Naoyuki Taniguchi from the Department of Biochemistry, Osaka University, Japan.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
Keywords
- Activator protein-1 (AP-1)
- Ageing
- Gene expression
- Nuclear factor-κB (NFκB)
- Skeletal muscle
- Superoxide dismutase