[3H]N-methylscopolamine binding to muscarinic receptors in intact adult rat brain cell aggregates

Jong Hwa Lee, Esam E. El-Fakahany

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Intact brain cell aggregates were dissociated from adult rat brains, by a simple sieving technique, and were used to study the binding characteristics of [3H]N-methylscopolamine to muscarinic acetylcholine receptors. The magnitude of binding of this ligand was related linearly to the amount of cell protein in the binding assay, with a high ratio of total to nonspecific binding. In addition, specific binding showed saturability and high affinity. Muscarinic receptor antagonists displaced specific [3H]N-methylscopolamine binding according to the law of mass-action, while it was possible to resolve displacement curves using receptor agonists into high- and low-affinity components. The results are discussed in terms of the usefulness of dissociated intact rat brain cells in studying muscarinic acetylcholine receptors in the central nervous system.

Original languageEnglish (US)
Pages (from-to)4299-4303
Number of pages5
JournalBiochemical Pharmacology
Volume34
Issue number24
DOIs
StatePublished - Dec 15 1985

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