Abstract
Intact brain cell aggregates were dissociated from adult rat brains, by a simple sieving technique, and were used to study the binding characteristics of [3H]N-methylscopolamine to muscarinic acetylcholine receptors. The magnitude of binding of this ligand was related linearly to the amount of cell protein in the binding assay, with a high ratio of total to nonspecific binding. In addition, specific binding showed saturability and high affinity. Muscarinic receptor antagonists displaced specific [3H]N-methylscopolamine binding according to the law of mass-action, while it was possible to resolve displacement curves using receptor agonists into high- and low-affinity components. The results are discussed in terms of the usefulness of dissociated intact rat brain cells in studying muscarinic acetylcholine receptors in the central nervous system.
Original language | English (US) |
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Pages (from-to) | 4299-4303 |
Number of pages | 5 |
Journal | Biochemical Pharmacology |
Volume | 34 |
Issue number | 24 |
DOIs | |
State | Published - Dec 15 1985 |
Bibliographical note
Funding Information:Acknowledgemenls-This research was supported in part by a UMAB graduates chool research award and a grant from the National Science Foundation (BNS-84-06357) to E.E.E. The authors are grateful to Ms. Anita Saulsbury for typing the manuscript and to Mr. Michael Gentry for his continuous help.