[3H]N-methylscopolamine binding to muscarinic receptors in intact adult rat brain cell aggregates

Jong Hwa Lee, Esam E. El-Fakahany

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Intact brain cell aggregates were dissociated from adult rat brains, by a simple sieving technique, and were used to study the binding characteristics of [3H]N-methylscopolamine to muscarinic acetylcholine receptors. The magnitude of binding of this ligand was related linearly to the amount of cell protein in the binding assay, with a high ratio of total to nonspecific binding. In addition, specific binding showed saturability and high affinity. Muscarinic receptor antagonists displaced specific [3H]N-methylscopolamine binding according to the law of mass-action, while it was possible to resolve displacement curves using receptor agonists into high- and low-affinity components. The results are discussed in terms of the usefulness of dissociated intact rat brain cells in studying muscarinic acetylcholine receptors in the central nervous system.

Original languageEnglish (US)
Pages (from-to)4299-4303
Number of pages5
JournalBiochemical Pharmacology
Issue number24
StatePublished - Dec 15 1985

Bibliographical note

Funding Information:
Acknowledgemenls-This research was supported in part by a UMAB graduates chool research award and a grant from the National Science Foundation (BNS-84-06357) to E.E.E. The authors are grateful to Ms. Anita Saulsbury for typing the manuscript and to Mr. Michael Gentry for his continuous help.


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