31P NMR studies of the solution structure and dynamics of nucleosomes and DNA

Leonard Klevan; Ian M. Armitage; Donald M. Crothers

doi:10.1093/nar/6.4.1607

³¹P NMR studies of the solution structure and dynamics of nucleosomes and DNA

Leonard Klevan, Ian M. Armitage, Donald M. Crothers

Biochemistry, Molecular Biology, and Biophysics (TMED)

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

³¹P NMR studies of 140 base pair DNA fragments in nucleosomes and free in solution show no detectable change in the internucleotide ³¹p chemical shift or linewidth when DNA is packaged into nucleosomes. Measurements of ³¹p spin-lattice relaxation times T₁ and ³¹p-{H} nuclear Overhauser enhancements revealed internal motion with a correlation time of about 4 × 10^-10 sec in double helical DNA, both free in solution and bound to nucleosomal core proteins. This result implies greater dynamic mobility in double helical DNA than has previously been supposed.

Original language	English (US)
Pages (from-to)	1607-1616
Number of pages	10
Journal	Nucleic acids research
Volume	6
Issue number	4
DOIs	https://doi.org/10.1093/nar/6.4.1607
State	Published - Apr 1979

Bibliographical note

Funding Information:
This research was supported by National Institutes of

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10.1093/nar/6.4.1607

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abstract = "31P NMR studies of 140 base pair DNA fragments in nucleosomes and free in solution show no detectable change in the internucleotide 31p chemical shift or linewidth when DNA is packaged into nucleosomes. Measurements of 31p spin-lattice relaxation times T1 and 31p-{H} nuclear Overhauser enhancements revealed internal motion with a correlation time of about 4 × 10-10 sec in double helical DNA, both free in solution and bound to nucleosomal core proteins. This result implies greater dynamic mobility in double helical DNA than has previously been supposed.",

author = "Leonard Klevan and Armitage, {Ian M.} and Crothers, {Donald M.}",

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AU - Armitage, Ian M.

AU - Crothers, Donald M.

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AB - 31P NMR studies of 140 base pair DNA fragments in nucleosomes and free in solution show no detectable change in the internucleotide 31p chemical shift or linewidth when DNA is packaged into nucleosomes. Measurements of 31p spin-lattice relaxation times T1 and 31p-{H} nuclear Overhauser enhancements revealed internal motion with a correlation time of about 4 × 10-10 sec in double helical DNA, both free in solution and bound to nucleosomal core proteins. This result implies greater dynamic mobility in double helical DNA than has previously been supposed.

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