31P NMR studies of intact anchorage-dependent mouse embryo fibroblasts

K. Ugurbil, D. L. Guernsey, T. R. Brown

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

31P NMR spectra of 'normal' and x-ray-transformed anchorage-dependent mouse embryo fibroblasts (C3H/10T 1/2 ) at 145.7 MHz and 37°C were obtained by using a solenoid coil probe with a sensitivity ~2.6 times higher than that of the standard Helmholtz coil probe. The cells were grown as mono-layers on the surface of microcarrier beads. Results show that these cells have low levels of NMR-detectable ADP, are highly impermeable to protons, are capable of maintaining high ATP levels in the absence of respiration, and, when ATP generation is blocked, ultimately hydrolyze their existing ATP to a nonphosphorylated product and P(i). Only small differences were observed between the spectra of 'normal' and transformed cells. However, the latter accumulated 2-deoxyglucose 6-phosphate at a faster rate.

Original languageEnglish (US)
Pages (from-to)4843-4847
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number8 I
DOIs
StatePublished - 1981
Externally publishedYes

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