31P-NMR spectroscopy and ultrastructural studies on nephrotoxicity of cyclosporine A

J. Ellermann, H. David, I. Marx, W. Gründer, H. Pfeifer, D. Scholz, K. Jung

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2 Scopus citations

Abstract

NMR-spectroscopy and electron microscopy were applied in order to find out whether nephrotoxic effects of cyclosporine A in combination with ischaemia on rat kidney are of significance for the energy metabolism of the organ. CSA was administered in daily doses of 15 mg/kg rat over 20 days. Use was made of the particular advantage of 31P-NMR-spectroscopy to follow up the dynamics of high-energy phosphate concentrations in the same tissue. Ultrastructural changes were observed in the region of the proximal tubule. Some mitochondria showed degenerative changes, others increased density of cristae. The total number of mitochondria was increased. This observation together with the coexistence of vacuolarly degenerated mitochondria and mitochondria with increased activity after CSA treatment is interpreted as expression of a compensatory mechanism that keeps constant the totality of high-energy phosphates despite damage to some mitochondria.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalExperimental Pathology
Volume32
Issue number2
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Keywords

  • cyclosporine A (CSA)
  • energy metabolism
  • ischaemia
  • kidney, toxic effects
  • nephrotoxicity
  • nuclear magnetic resonance (NMR) spectroscopy, in vivo
  • rat
  • ultrastructure

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