31P NMR measurement of ATP synthesis rate in perfused intact rat hearts

P. Kingsley-Hickman, E. Y. Sako, P. A. Andreone, J. A. St. Cyr, S. Michurski, J. E. Foker, A. H.L. From, M. Petein, K. Ugurbil

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29 Scopus citations

Abstract

Using 31P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 ± 2.5 (SE) × 103 mmHg · min-1 and consuming O2; at a rate of 35 ± 2 μmol O2 · min-1. (g dry wt)-1, at 37°C. This rate was 7.2 ± 0.9 μmol · s-1 · (g dry wt)-1 and was related to the rate of oxygen atom consumption by a ratio of 6.3 ± 0.9. These date show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.

Original languageEnglish (US)
Pages (from-to)159-163
Number of pages5
JournalFEBS Letters
Volume198
Issue number1
DOIs
StatePublished - Mar 17 1986

Keywords

  • ( Rat myocardium)
  • ATPase
  • P-NMR
  • Saturation transfer

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