1H NMR Detection of Vitamin C in Human Brain In Vivo

Melissa Terpstra, Rolf Gruetter

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


Vitamin C (ascorbate) is well established as an essential nutrient that functions as an antioxidant. Since it is present in the human brain at detectable concentrations, this study was designed to detect and quantify ascorbate in the human brain in vivo using 1H NMR spectroscopy (MRS). Ascorbate was consistently detected in all five study subjects, and was measured using MEGA-PRESS difference editing. The in vivo resonance pattern was consistent with that of ascorbate based on position, line width, peak pattern, and relative intensity. Metabolites with a potential for coediting were assessed using phantom solutions. The putative resonances of myo-inositol, lactate, glycerophosphocholine, phosphocholine, and phosphoethanolamine were detected at positions distinct from those of ascorbate. This study represents the first in vivo detection of vitamin C in the human brain using 1H MRS. A concentration of 1.3 ± 0.3 μmol/g (mean ± SD, N = 4) was estimated.

Original languageEnglish (US)
Pages (from-to)225-229
Number of pages5
JournalMagnetic resonance in medicine
Issue number2
StatePublished - Feb 2004


  • Ascorbic acid
  • Brain
  • Human
  • Magnetic resonance spectroscopy
  • Vitamin C


Dive into the research topics of '1H NMR Detection of Vitamin C in Human Brain In Vivo'. Together they form a unique fingerprint.

Cite this