1H NMR and NOE Studies of the Purple Acid Phosphatases from Porcine Uterus and Bovine Spleen

Zhigang Wang, Li June Ming, Lawrence Que, John B. Vincent, Michael W. Crowder, Bruce A. Averill

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Abstract

The diiron active sites of the purple acid phosphatases from porcine uterus (also called uteroferrin, Uf) and bovine spleen (BSPAP) and their complexes with tungstate are compared by 1H NMR and NOE techniques. The paramagnetically shifted features of the 1H NMR spectrum of reduced BSPAP are similar to those of reduced Uf, while the spectra of the tungstate complexes are almost identical. These observations suggest that the two active sites are quite similar, in agreement with the >90% sequence homology found in the two enzymes. Nuclear Overhauser effect (NOE) experiments on the His N-H resonances show that the Fe(III)-His residue is Ne-coordinated, while the Fe(II)-His is Hj-coordinated in both enzymes. On the basis of the above NMR and NOE results, our previously proposed model for the dinuclear iron active site of Uf [Scarrow, R.C., Pyrz, J.W., & Que, L., Jr. (1990) J. Am. Chem. Soc. 112, 657-665] is corroborated, refined, and found to represent the diiron center of BSPAP as well.

Original languageEnglish (US)
Pages (from-to)5263-5268
Number of pages6
JournalBiochemistry
Volume31
Issue number23
DOIs
StatePublished - Feb 1 1992

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    Wang, Z., Ming, L. J., Que, L., Vincent, J. B., Crowder, M. W., & Averill, B. A. (1992). 1H NMR and NOE Studies of the Purple Acid Phosphatases from Porcine Uterus and Bovine Spleen. Biochemistry, 31(23), 5263-5268. https://doi.org/10.1021/bi00138a004