1H MRS detection of glycine residue of reduced glutathione in vivo

Lana G. Kaiser, Małgorzata Marjańska, Gerald B. Matson, Isabelle Iltis, Seth D. Bush, Brian J. Soher, Susanne Mueller, Karl Young

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Glutathione (GSH) is a powerful antioxidant found inside different kinds of cells, including those of the central nervous system. Detection of GSH in the human brain using 1H MR spectroscopy is hindered by low concentration and spectral overlap with other metabolites. Previous MRS methods focused mainly on the detection of the cysteine residue (GSH-Cys) via editing schemes. This study focuses on the detection of the glycine residue (GSH-Gly), which is overlapped by glutamate and glutamine (Glx) under physiological pH and temperature. The first goal of the study was to obtain the spectral parameters for characterization of the GSH-Gly signal under physiological conditions. The second goal was to investigate a new method of separating GSH-Gly from Glx in vivo. The characterization of the signal was carried out by utilization of numerical simulations as well as experiments over a wide range of magnetic fields (4.0-14 T). The proposed separation scheme utilizes J-difference editing to quantify the Glx contribution to separate it from the GSH-Gly signal. The presented method retains 100% of the GSH-Gly signal. The overall increase in signal to noise ratio of the targeted resonance is calculated to yield a significant SNR improvement compared to previously used methods that target GSH-Cys residue. This allows shorter acquisition times for in vivo human clinical studies.

Original languageEnglish (US)
Pages (from-to)259-266
Number of pages8
JournalJournal of Magnetic Resonance
Volume202
Issue number2
DOIs
StatePublished - Feb 1 2010

Keywords

  • Brain
  • MEGA-PRESS
  • PRESS + 4
  • Spectral editing

Fingerprint Dive into the research topics of '<sup>1</sup>H MRS detection of glycine residue of reduced glutathione in vivo'. Together they form a unique fingerprint.

  • Cite this

    Kaiser, L. G., Marjańska, M., Matson, G. B., Iltis, I., Bush, S. D., Soher, B. J., Mueller, S., & Young, K. (2010). 1H MRS detection of glycine residue of reduced glutathione in vivo. Journal of Magnetic Resonance, 202(2), 259-266. https://doi.org/10.1016/j.jmr.2009.11.013