19F-NMR studies of retinol transfer between cellular retinol binding proteins and phospholipid vesicles

Ding Rong, Chan Lan S. Lin, D. Andre D'Avignon, Allen J. Lovey, Michael Rosenberger, Ellen Li

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol and intracellular trafficking of retinol between sites of metabolic processing. 19F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Differences in how readily protein-bound retinol is released to lipid bilayers may lead to differences in how these two proteins modulate intracellular retinol metabolism.

Original languageEnglish (US)
Pages (from-to)116-120
Number of pages5
JournalFEBS Letters
Volume402
Issue number2-3
DOIs
StatePublished - Jan 27 1997
Externally publishedYes

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Keywords

  • Cellular retinol binding protein
  • Cellular retinol binding protein II
  • F-NMR
  • Liposome
  • Retinol

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