The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol and intracellular trafficking of retinol between sites of metabolic processing. 19F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Differences in how readily protein-bound retinol is released to lipid bilayers may lead to differences in how these two proteins modulate intracellular retinol metabolism.
Bibliographical noteFunding Information:
This work was supported by National Institutes of Health Grant DK 40172. NMR spectra were collected at the Washington University High Resolution NMR Facility which is funded in part through NIH Biomedical Research support shared instrument grants RR02004, RR05018 and RR07155. E.L. is a Burroughs Wellcome Scholar in Toxicology. We thank Marc Levin and David Cistola for many helpful discussions.
- Cellular retinol binding protein
- Cellular retinol binding protein II