14C-glycine uptake by fish scales: Refinement of a growth index and effects of a protein-synthesis inhibitor

Edward M. Goolish, Ira R. Adelman

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

In vitro 14C-glycine uptake by the scales of bluegill Lepomis macrochirus was sensitive to the pH of the incubation medium; maximum uptake occurred at approximately pH 7.7. Cycloheximide, an inhibitor of protein synthesis, reduced glycine uptake by up to 90%, providing further evidence for the involvement of glycine uptake in the process of protein synthesis. The minimum duration of handling stress that resulted in a significant change in glycine uptake was 60 minutes; after 240 minutes of continuous stress, glycine uptake was reduced 62% from control values. Diel sampling of laboratory fish indicated no daily rhythm in glycine uptake and demonstrated a significant correlation (r = 0.837) between glycine uptake and fish growth rate as estimated over a 2-week interval. The results of these experiments suggest that glycine uptake by scales can be a reliable index of fish growth or state of stress.

Original languageEnglish (US)
Pages (from-to)647-652
Number of pages6
JournalTransactions of the American Fisheries Society
Volume112
Issue number5
DOIs
StatePublished - Sep 1 1983

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