Summary of the first round analyses of the Second International Swine CD Workshop

A. Saalmüller, T. Pauly, B. Aasted, K. T. Jensen, D. H. Sachs, S. Arn, W. C. Davis, Y. H. Park, K. McCullough, A. Summerfield, M. Murtaugh, M. S. Pampusch, K. D. Burger, J. Laber, J. Nielsen, M. D. Pescovitz, C. Stokes, K. Haverson, P. Boyd, J. K. Lunney

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The reactivity of 176 monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, together with 19 internal standards, was analyzed by flow cytometry on 16 different cell types as a means of establishing the proper cell subset for later detailed clustering analyses. The exact CD subset reactivity of the 19 internal standard mAb had been characterized in the First International Swine CD Workshop. The flow cytometric analyses resulted in 40 data sets which were then subjected to statistical clustering using the Leukocyte Typing Database IV (LTDB4) software. As result of this work, 22 clusters were defined. After review of these results, panels of mAb from the defined first round clusters were assigned to cell subsets. The respective mAb in those first round clusters were then distributed to subset group researchers for further examination during the second round of the workshop.

Original languageEnglish (US)
Pages (from-to)237-249
Number of pages13
JournalVeterinary immunology and immunopathology
Volume60
Issue number3-4
DOIs
StatePublished - Jan 30 1998

Keywords

  • Clustering analysis
  • Flow cytometry
  • Monoclonal antibodies

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