Successful sperm storage for 28 years

Joseph Feldschuh, James Brassel, Nancy Durso, Allen Levine

Research output: Contribution to journalArticlepeer-review

83 Scopus citations


Objective: To report on two instances of successful long-term cryopreservation resulting in live births. Design: Case report. Setting: The patient stored his sperm at a private sperm banking facility, and his partner underwent artificial insemination at an obstetrics/gynecology practice for one pregnancy and at a fertility center for the other. Patient(s): One man who stored his sperm before treatment for cancer. Intervention(s): Storage of sperm under liquid nitrogen in a carousel canister system. Intrauterine insemination in which the semen was thawed, washed with human tubal fluid (HTF), and inseminated. Main Outcome Measure(s): Successful pregnancy resulting in live birth. Result(s): Artificial insemination with semen cryopreserved for 21 and 28 years resulted in two live births. Conclusion(s): This case report describes the to-date longest known successful cryopreservation of sperm, with two live births resulting from IUI. Successful long-term semen storage can be very beneficial for men facing impaired fertility or sterility early in life, so that they have sufficient time to make appropriate family planning decisions.

Original languageEnglish (US)
Pages (from-to)1017.e3-1017.e4
JournalFertility and Sterility
Issue number4
StatePublished - Oct 2005


  • Cryopreservation
  • Intrauterine insemination
  • Semen storage


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