Successful long-term cryopreservation of neonatal rat islet tissue

W. D. Payne, D. E R Sutherland, A. J. Matas, J. S. Najarian

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Neonatal rat pancreatic tissue was frozen to -196 °C using Me2SO as a cryoprotectant and a slow freezing rate to -70 °C followed by immersion in liquid nitrogen. Rapid thawing was used. Viability was demonstrated by successful transplantation to streptozotocin-induced diabetic recipients. Long-term preservation, up to 4 weeks, did not demonstrably affect viability. Cryopreservation techniques may afford a method for providing a diabetic recipient the opportunity to receive a large quantity of pooled islet tissue from well-matched donors.

Original languageEnglish (US)
Pages (from-to)226-229
Number of pages4
Issue number2
StatePublished - Apr 1983


Dive into the research topics of 'Successful long-term cryopreservation of neonatal rat islet tissue'. Together they form a unique fingerprint.

Cite this