Successful donor cell engraftment in a recipient of bone marrow from a cadaveric donor

Bruce R Blazar, L. C. Lasky, J. P. Perentesis, K. V. Watson, S. E. Steinberg, A. H. Filipovich, Harry T Orr, N. K. Ramsay

Research output: Contribution to journalArticle

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Abstract

A 12-year-old male with acute lymphocytic leukemia received donor bone marrow from his histocompatible father whose marrow was harvested 40 minutes postmortem after he suffered a myocardial infarction. The marrow was stored in liquid nitrogen for 17 days prior to infusion into the recipient. Trypan blue viability was >99% for the fresh marrow. Progenitor cell assays revealed that 20% of the CFU-MIX, 16% of the BFU-E, 10% of the CFU-E, and 17% of the CFU-GM were spared during the cryopreservation period. Posttransplantation, the recipient had a leukocyte count >103 μL by day 26. Southern blotting analysis documented the donor origin of the peripheral blood mononuclear cells and granulocytes isolated 46 days posttransplantation. Unfortunately, the patient died of complications relating to graft-v-host disease 67 days following transplantation. This case demonstrates the feasibility of cadaveric marrow as a source of donor cells and is the first reported case of documented leukocyte engraftment in a recipient of cadaveric marrow.

Original languageEnglish (US)
Pages (from-to)1655-1660
Number of pages6
JournalBlood
Volume67
Issue number6
StatePublished - Oct 8 1986

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Transplantation (surgical)
Trypan Blue
Liquid nitrogen
Grafts
Assays
Bone
Blood
Bone Marrow
Tissue Donors
Erythroid Precursor Cells
Granulocyte-Macrophage Progenitor Cells
Cryopreservation
Southern Blotting
Leukocyte Count
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Granulocytes
Fathers
E 10
Blood Cells
Leukocytes

Cite this

Blazar, B. R., Lasky, L. C., Perentesis, J. P., Watson, K. V., Steinberg, S. E., Filipovich, A. H., ... Ramsay, N. K. (1986). Successful donor cell engraftment in a recipient of bone marrow from a cadaveric donor. Blood, 67(6), 1655-1660.

Successful donor cell engraftment in a recipient of bone marrow from a cadaveric donor. / Blazar, Bruce R; Lasky, L. C.; Perentesis, J. P.; Watson, K. V.; Steinberg, S. E.; Filipovich, A. H.; Orr, Harry T; Ramsay, N. K.

In: Blood, Vol. 67, No. 6, 08.10.1986, p. 1655-1660.

Research output: Contribution to journalArticle

Blazar, BR, Lasky, LC, Perentesis, JP, Watson, KV, Steinberg, SE, Filipovich, AH, Orr, HT & Ramsay, NK 1986, 'Successful donor cell engraftment in a recipient of bone marrow from a cadaveric donor', Blood, vol. 67, no. 6, pp. 1655-1660.
Blazar BR, Lasky LC, Perentesis JP, Watson KV, Steinberg SE, Filipovich AH et al. Successful donor cell engraftment in a recipient of bone marrow from a cadaveric donor. Blood. 1986 Oct 8;67(6):1655-1660.
Blazar, Bruce R ; Lasky, L. C. ; Perentesis, J. P. ; Watson, K. V. ; Steinberg, S. E. ; Filipovich, A. H. ; Orr, Harry T ; Ramsay, N. K. / Successful donor cell engraftment in a recipient of bone marrow from a cadaveric donor. In: Blood. 1986 ; Vol. 67, No. 6. pp. 1655-1660.
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