Successful cryopreservation of coral larvae using vitrification and laser warming

Jonathan Daly, Nikolas Zuchowicz, C. Isabel Nuñez Lendo, Kanav Khosla, Claire Lager, E. Michael Henley, John C Bischof, F. W. Kleinhans, Chiahsin Lin, Esther C. Peters, Mary Hagedorn

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Climate change has increased the incidence of coral bleaching events, resulting in the loss of ecosystem function and biodiversity on reefs around the world. As reef degradation accelerates, the need for innovative restoration tools has become acute. Despite past successes with ultra-low temperature storage of coral sperm to conserve genetic diversity, cryopreservation of larvae has remained elusive due to their large volume, membrane complexity, and sensitivity to chilling injury. Here we show for the first time that coral larvae can survive cryopreservation and resume swimming after warming. Vitrification in a 3.5 M cryoprotectant solution (10% v/v propylene glycol, 5% v/v dimethyl sulfoxide, and 1 M trehalose in phosphate buffered saline) followed by warming at a rate of approximately 4,500,000 °C/min with an infrared laser resulted in up to 43% survival of Fungia scutaria larvae on day 2 post-fertilization. Surviving larvae swam and continued to develop for at least 12 hours after laser-warming. This technology will enable biobanking of coral larvae to secure biodiversity, and, if managed in a high-throughput manner where millions of larvae in a species are frozen at one time, could become an invaluable research and conservation tool to help restore and diversify wild reef habitats.

Original languageEnglish (US)
Article number15714
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

Vitrification
Anthozoa
Cryopreservation
Larva
Lasers
Biodiversity
Ecosystem
Propylene Glycol
Trehalose
Climate Change
Dimethyl Sulfoxide
Fertilization
Spermatozoa
Phosphates
Technology
Temperature
Membranes
Incidence
Wounds and Injuries
Research

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Daly, J., Zuchowicz, N., Nuñez Lendo, C. I., Khosla, K., Lager, C., Henley, E. M., ... Hagedorn, M. (2018). Successful cryopreservation of coral larvae using vitrification and laser warming. Scientific reports, 8(1), [15714]. https://doi.org/10.1038/s41598-018-34035-0

Successful cryopreservation of coral larvae using vitrification and laser warming. / Daly, Jonathan; Zuchowicz, Nikolas; Nuñez Lendo, C. Isabel; Khosla, Kanav; Lager, Claire; Henley, E. Michael; Bischof, John C; Kleinhans, F. W.; Lin, Chiahsin; Peters, Esther C.; Hagedorn, Mary.

In: Scientific reports, Vol. 8, No. 1, 15714, 01.12.2018.

Research output: Contribution to journalArticle

Daly, J, Zuchowicz, N, Nuñez Lendo, CI, Khosla, K, Lager, C, Henley, EM, Bischof, JC, Kleinhans, FW, Lin, C, Peters, EC & Hagedorn, M 2018, 'Successful cryopreservation of coral larvae using vitrification and laser warming', Scientific reports, vol. 8, no. 1, 15714. https://doi.org/10.1038/s41598-018-34035-0
Daly J, Zuchowicz N, Nuñez Lendo CI, Khosla K, Lager C, Henley EM et al. Successful cryopreservation of coral larvae using vitrification and laser warming. Scientific reports. 2018 Dec 1;8(1). 15714. https://doi.org/10.1038/s41598-018-34035-0
Daly, Jonathan ; Zuchowicz, Nikolas ; Nuñez Lendo, C. Isabel ; Khosla, Kanav ; Lager, Claire ; Henley, E. Michael ; Bischof, John C ; Kleinhans, F. W. ; Lin, Chiahsin ; Peters, Esther C. ; Hagedorn, Mary. / Successful cryopreservation of coral larvae using vitrification and laser warming. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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