Subunit vaccine formulations based on recombinant envelope proteins of Chikungunya virus elicit balanced Th1/Th2 response and virus-neutralizing antibodies in mice

Mohsin Khan, Rekha Dhanwani, Putcha Venkata Lakshamana Rao, Manmohan Parida

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

The recent resurgence of Chikungunya virus in India and Indian Ocean Islands with unusual clinical severity is a matter of great public health concern. Despite the fact that CHIKV resurgence is associated with epidemic of unprecedented magnitude, none of the vaccine candidate has been approved so far. The envelope protein E1 and E2 being the major immunodominant structural proteins with crucial role in virus attachment and entry, can prove to be potential vaccine candidates. In the present study, the immunogenic potential of bacterially expressed CHIKE1 and CHIKE2 recombinant proteins along with various adjuvants is reported. Assessment of the protective efficacy of both the vaccine formulations was further confirmed by both in vitro and in vivo neutralisation tests. Splenocytes from immunized mice, cultured in vitro when stimulated with the vaccine antigens revealed induction of very high levels of both pro- and anti- inflammatory cytokines indicating a balance of Th1 and Th2 response.

Original languageEnglish (US)
Pages (from-to)236-246
Number of pages11
JournalVirus research
Volume167
Issue number2
DOIs
StatePublished - Aug 2012

Bibliographical note

Funding Information:
M.K. is a recipient of DRDO senior research fellowship. The work was supported by the fund of DRDO, Ministry of Defence, Government of India . We also thank Dr. R. Vijayaraghavan, Director, DRDE for his support and providing basic infrastructure to carry out this study.

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

Keywords

  • Cell mediated response
  • Chikungunya vaccine
  • Cytokine profiling
  • Envelope protein
  • Humoral response

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