TY - JOUR
T1 - Study of the irreversible adsorption of proteins on polybutadiene-coated zirconia
AU - Sun, Lifang
AU - McCormick, Alon V.
AU - Carr, Peter W.
N1 - Funding Information:
We would like to thank the National Institute of Health and National Science Foundation (CHE9107029) for support of this work .
PY - 1994/1/14
Y1 - 1994/1/14
N2 - The cause of irreversible adsorption of proteins on polybutadiene-coated zirconia is investigated by comparing the chromatographic properties of polybutadiene-coated zirconia with that of other reversed-phase packing materials such as bonded phase silica, polybutadiene-coated alumina and polybutadiene-coated silica. We find that the polybutadiene-coated zirconia has a micropore size distribution similar to that of the polybutadiene-coated alumina, from which some proteins can be eluted. Thus, the irreversible adsorption of proteins on polybutadiene-coated zirconia is not caused by entrapment of proteins in the micropores of the packing. The high hydrophobicity of the polybutadiene coating and the strong Lewis acid sites on the zirconia surface cause strong interactions between proteins and the stationary phase, the combination of which lead to irreversible adsorption of proteins on polybutadiene-coated zirconia.
AB - The cause of irreversible adsorption of proteins on polybutadiene-coated zirconia is investigated by comparing the chromatographic properties of polybutadiene-coated zirconia with that of other reversed-phase packing materials such as bonded phase silica, polybutadiene-coated alumina and polybutadiene-coated silica. We find that the polybutadiene-coated zirconia has a micropore size distribution similar to that of the polybutadiene-coated alumina, from which some proteins can be eluted. Thus, the irreversible adsorption of proteins on polybutadiene-coated zirconia is not caused by entrapment of proteins in the micropores of the packing. The high hydrophobicity of the polybutadiene coating and the strong Lewis acid sites on the zirconia surface cause strong interactions between proteins and the stationary phase, the combination of which lead to irreversible adsorption of proteins on polybutadiene-coated zirconia.
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U2 - 10.1016/0021-9673(94)80037-5
DO - 10.1016/0021-9673(94)80037-5
M3 - Article
C2 - 8118552
AN - SCOPUS:0028351874
VL - 658
SP - 465
EP - 473
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 2
ER -