Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae

Javier Bezos; Julio Álvarez; Inmaculada Moreno; Lucía de Juan; Beatriz Romero; Sabrina Rodríguez; Mercedes Domínguez; Alfredo Toraño; Ana Mateos; Lucas Domínguez; Alicia Aranaz

doi:10.1016/j.rvsc.2011.05.020

Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae

Javier Bezos, Julio Álvarez, Inmaculada Moreno, Lucía de Juan, Beatriz Romero, Sabrina Rodríguez, Mercedes Domínguez, Alfredo Toraño, Ana Mateos, Lucas Domínguez, Alicia Aranaz

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Tuberculosis in goats caused by Mycobacterium bovis and Mycobacterium caprae has noteworthy sanitary and economic implications. Current diagnostic assays are based on cellular immunity and although they have demonstrated a high sensitivity, some animals remain undetected. In the present study, flow cytometry has been used to determine changes in CD4+, CD8+ and CD25+ T cell populations in peripheral blood from naturally infected goats. Proportion of lymphocytes producing PPD-specific interferon-gamma (IFN-γ) was calculated and an ELISA for detection of PPD-specific IFN-γ was performed to measure the cytokine in plasma. The infected goats showed percentages of CD4+ T cells between 27.31% and 47.23% and there were not significant differences (p= 0.113) with the non-infected control goats although the mean percentage was lower in this group. Regarding CD8+ T cells, a higher percentage was observed in healthy goats compared to controls (p=0.081). The mean percentage of lymphocytes expressing CD25 without antigen stimulation (30.65 ± 3.91) was higher in lesion and/or culture-positive animals than in the controls (21.84 ± 1.21; p= 0.053). The percentage of CD4+/IFN+ T cell population stimulated with bovine PPD was a reliable marker of infection, since the mean percentage in the infected goats was significantly higher than in the controls (p< 0.05). Tuberculosis in goats caused by M. caprae induced changes in cellular populations similar to those described for M. bovis in cattle.

Original language	English (US)
Pages (from-to)	163-167
Number of pages	5
Journal	Research in veterinary science
Volume	93
Issue number	1
DOIs	https://doi.org/10.1016/j.rvsc.2011.05.020
State	Published - Aug 2012
Externally published	Yes

Bibliographical note

Funding Information:
This research was funded by project AGL2006-06206 of the Spanish Ministry of Science and Technology and the Ministry of Environment and Rural and Marine Affairs . J. Bezos is the recipient of a research contract assigned by the Spanish Ministry of Science and Innovation and the European Social Fund. We would like to thank to National and Regional Animal Health Authorities for their continuous encouragement. We are also grateful to F. Lozano, N. Moya and S. González for technical assistance.

Keywords

Flow cytometry
Goats
Interferon-gamma
Tuberculosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1016/j.rvsc.2011.05.020

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Bezos, J., Álvarez, J., Moreno, I., de Juan, L., Romero, B., Rodríguez, S., Domínguez, M., Toraño, A., Mateos, A., Domínguez, L., & Aranaz, A. (2012). Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae. Research in veterinary science, 93(1), 163-167. https://doi.org/10.1016/j.rvsc.2011.05.020

Bezos, J, Álvarez, J, Moreno, I, de Juan, L, Romero, B, Rodríguez, S, Domínguez, M, Toraño, A, Mateos, A, Domínguez, L & Aranaz, A 2012, 'Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae', Research in veterinary science, vol. 93, no. 1, pp. 163-167. https://doi.org/10.1016/j.rvsc.2011.05.020

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abstract = "Tuberculosis in goats caused by Mycobacterium bovis and Mycobacterium caprae has noteworthy sanitary and economic implications. Current diagnostic assays are based on cellular immunity and although they have demonstrated a high sensitivity, some animals remain undetected. In the present study, flow cytometry has been used to determine changes in CD4+, CD8+ and CD25+ T cell populations in peripheral blood from naturally infected goats. Proportion of lymphocytes producing PPD-specific interferon-gamma (IFN-γ) was calculated and an ELISA for detection of PPD-specific IFN-γ was performed to measure the cytokine in plasma. The infected goats showed percentages of CD4+ T cells between 27.31% and 47.23% and there were not significant differences (p= 0.113) with the non-infected control goats although the mean percentage was lower in this group. Regarding CD8+ T cells, a higher percentage was observed in healthy goats compared to controls (p=0.081). The mean percentage of lymphocytes expressing CD25 without antigen stimulation (30.65 ± 3.91) was higher in lesion and/or culture-positive animals than in the controls (21.84 ± 1.21; p= 0.053). The percentage of CD4+/IFN+ T cell population stimulated with bovine PPD was a reliable marker of infection, since the mean percentage in the infected goats was significantly higher than in the controls (p< 0.05). Tuberculosis in goats caused by M. caprae induced changes in cellular populations similar to those described for M. bovis in cattle.",

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note = "Funding Information: This research was funded by project AGL2006-06206 of the Spanish Ministry of Science and Technology and the Ministry of Environment and Rural and Marine Affairs . J. Bezos is the recipient of a research contract assigned by the Spanish Ministry of Science and Innovation and the European Social Fund. We would like to thank to National and Regional Animal Health Authorities for their continuous encouragement. We are also grateful to F. Lozano, N. Moya and S. Gonz{\'a}lez for technical assistance.",

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AU - Bezos, Javier

AU - Álvarez, Julio

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AU - de Juan, Lucía

AU - Romero, Beatriz

AU - Rodríguez, Sabrina

AU - Domínguez, Mercedes

AU - Toraño, Alfredo

AU - Mateos, Ana

AU - Domínguez, Lucas

AU - Aranaz, Alicia

N1 - Funding Information: This research was funded by project AGL2006-06206 of the Spanish Ministry of Science and Technology and the Ministry of Environment and Rural and Marine Affairs . J. Bezos is the recipient of a research contract assigned by the Spanish Ministry of Science and Innovation and the European Social Fund. We would like to thank to National and Regional Animal Health Authorities for their continuous encouragement. We are also grateful to F. Lozano, N. Moya and S. González for technical assistance.

PY - 2012/8

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N2 - Tuberculosis in goats caused by Mycobacterium bovis and Mycobacterium caprae has noteworthy sanitary and economic implications. Current diagnostic assays are based on cellular immunity and although they have demonstrated a high sensitivity, some animals remain undetected. In the present study, flow cytometry has been used to determine changes in CD4+, CD8+ and CD25+ T cell populations in peripheral blood from naturally infected goats. Proportion of lymphocytes producing PPD-specific interferon-gamma (IFN-γ) was calculated and an ELISA for detection of PPD-specific IFN-γ was performed to measure the cytokine in plasma. The infected goats showed percentages of CD4+ T cells between 27.31% and 47.23% and there were not significant differences (p= 0.113) with the non-infected control goats although the mean percentage was lower in this group. Regarding CD8+ T cells, a higher percentage was observed in healthy goats compared to controls (p=0.081). The mean percentage of lymphocytes expressing CD25 without antigen stimulation (30.65 ± 3.91) was higher in lesion and/or culture-positive animals than in the controls (21.84 ± 1.21; p= 0.053). The percentage of CD4+/IFN+ T cell population stimulated with bovine PPD was a reliable marker of infection, since the mean percentage in the infected goats was significantly higher than in the controls (p< 0.05). Tuberculosis in goats caused by M. caprae induced changes in cellular populations similar to those described for M. bovis in cattle.

AB - Tuberculosis in goats caused by Mycobacterium bovis and Mycobacterium caprae has noteworthy sanitary and economic implications. Current diagnostic assays are based on cellular immunity and although they have demonstrated a high sensitivity, some animals remain undetected. In the present study, flow cytometry has been used to determine changes in CD4+, CD8+ and CD25+ T cell populations in peripheral blood from naturally infected goats. Proportion of lymphocytes producing PPD-specific interferon-gamma (IFN-γ) was calculated and an ELISA for detection of PPD-specific IFN-γ was performed to measure the cytokine in plasma. The infected goats showed percentages of CD4+ T cells between 27.31% and 47.23% and there were not significant differences (p= 0.113) with the non-infected control goats although the mean percentage was lower in this group. Regarding CD8+ T cells, a higher percentage was observed in healthy goats compared to controls (p=0.081). The mean percentage of lymphocytes expressing CD25 without antigen stimulation (30.65 ± 3.91) was higher in lesion and/or culture-positive animals than in the controls (21.84 ± 1.21; p= 0.053). The percentage of CD4+/IFN+ T cell population stimulated with bovine PPD was a reliable marker of infection, since the mean percentage in the infected goats was significantly higher than in the controls (p< 0.05). Tuberculosis in goats caused by M. caprae induced changes in cellular populations similar to those described for M. bovis in cattle.

KW - Flow cytometry

KW - Goats

KW - Interferon-gamma

KW - Tuberculosis

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Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae

Abstract

Bibliographical note

Keywords

UN SDGs

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