Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp. KRS101

Natarajan Velmurugan; Yesupatham Sathishkumar; Sung Sun Yim; Yang Soo Lee; Min S. Park; Ji Won Yang; Ki Jun Jeong

doi:10.1016/j.biortech.2014.03.017

Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp. KRS101

Natarajan Velmurugan, Yesupatham Sathishkumar, Sung Sun Yim, Yang Soo Lee, Min S. Park, Ji Won Yang, Ki Jun Jeong

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Transmission electron, confocal microscopy and FACS in conjunction with two different lipophilic fluorescent dyes, BODIPY 505/515 and Nile Red were used to describe the cellular development and lipid bodies formation in Aurantiochytrium sp. KRS101. TEM results revealed that multi-cellular spores were appeared in sporangium during early-exponential phase, and spores were matured in mid-exponential phase followed by release of spores from sporangium in late-exponential phase. TEM and FACS analyses proved that lipid bodies appeared, developed and degenerated in mid-exponential, early- and late-stationary phases, respectively. The staining results in FACS indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies than Nile Red. FACS based single cell sorting also showed healthy growth for BODIPY 505/515 stained cells than Nile Red stained cells. In addition, a quantitative baseline was established either for cell growth and/or lipid accumulation based on cell count, fatty acid contents/composition, and sectional/confocal images of KRS101.

Original language	English (US)
Pages (from-to)	149-154
Number of pages	6
Journal	Bioresource Technology
Volume	161
DOIs	https://doi.org/10.1016/j.biortech.2014.03.017
State	Published - Jun 2014

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Science, ICT and Future Planning (MSIP) (ABC-2013-057282). N. Velmurugan was supported by the BK21 Post-Doctoral Research Fund and MSP was partially supported by Brain Pool Program of Korea.

Keywords

Aurantiochytrium sp.
BODIPY 505/515
Fluorescence-activated cell sorting
Nile Red
TEM analysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2014.03.017

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title = "Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp. KRS101",

abstract = "Transmission electron, confocal microscopy and FACS in conjunction with two different lipophilic fluorescent dyes, BODIPY 505/515 and Nile Red were used to describe the cellular development and lipid bodies formation in Aurantiochytrium sp. KRS101. TEM results revealed that multi-cellular spores were appeared in sporangium during early-exponential phase, and spores were matured in mid-exponential phase followed by release of spores from sporangium in late-exponential phase. TEM and FACS analyses proved that lipid bodies appeared, developed and degenerated in mid-exponential, early- and late-stationary phases, respectively. The staining results in FACS indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies than Nile Red. FACS based single cell sorting also showed healthy growth for BODIPY 505/515 stained cells than Nile Red stained cells. In addition, a quantitative baseline was established either for cell growth and/or lipid accumulation based on cell count, fatty acid contents/composition, and sectional/confocal images of KRS101.",

keywords = "Aurantiochytrium sp., BODIPY 505/515, Fluorescence-activated cell sorting, Nile Red, TEM analysis",

author = "Natarajan Velmurugan and Yesupatham Sathishkumar and Yim, {Sung Sun} and Lee, {Yang Soo} and Park, {Min S.} and Yang, {Ji Won} and Jeong, {Ki Jun}",

note = "Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Science, ICT and Future Planning (MSIP) (ABC-2013-057282). N. Velmurugan was supported by the BK21 Post-Doctoral Research Fund and MSP was partially supported by Brain Pool Program of Korea. ",

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AU - Yim, Sung Sun

AU - Lee, Yang Soo

AU - Park, Min S.

AU - Yang, Ji Won

AU - Jeong, Ki Jun

N1 - Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Science, ICT and Future Planning (MSIP) (ABC-2013-057282). N. Velmurugan was supported by the BK21 Post-Doctoral Research Fund and MSP was partially supported by Brain Pool Program of Korea.

PY - 2014/6

Y1 - 2014/6

N2 - Transmission electron, confocal microscopy and FACS in conjunction with two different lipophilic fluorescent dyes, BODIPY 505/515 and Nile Red were used to describe the cellular development and lipid bodies formation in Aurantiochytrium sp. KRS101. TEM results revealed that multi-cellular spores were appeared in sporangium during early-exponential phase, and spores were matured in mid-exponential phase followed by release of spores from sporangium in late-exponential phase. TEM and FACS analyses proved that lipid bodies appeared, developed and degenerated in mid-exponential, early- and late-stationary phases, respectively. The staining results in FACS indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies than Nile Red. FACS based single cell sorting also showed healthy growth for BODIPY 505/515 stained cells than Nile Red stained cells. In addition, a quantitative baseline was established either for cell growth and/or lipid accumulation based on cell count, fatty acid contents/composition, and sectional/confocal images of KRS101.

AB - Transmission electron, confocal microscopy and FACS in conjunction with two different lipophilic fluorescent dyes, BODIPY 505/515 and Nile Red were used to describe the cellular development and lipid bodies formation in Aurantiochytrium sp. KRS101. TEM results revealed that multi-cellular spores were appeared in sporangium during early-exponential phase, and spores were matured in mid-exponential phase followed by release of spores from sporangium in late-exponential phase. TEM and FACS analyses proved that lipid bodies appeared, developed and degenerated in mid-exponential, early- and late-stationary phases, respectively. The staining results in FACS indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies than Nile Red. FACS based single cell sorting also showed healthy growth for BODIPY 505/515 stained cells than Nile Red stained cells. In addition, a quantitative baseline was established either for cell growth and/or lipid accumulation based on cell count, fatty acid contents/composition, and sectional/confocal images of KRS101.

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Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp. KRS101

Abstract

Bibliographical note

Keywords

UN SDGs

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