Studies on the regulation of colicin Ib synthesis - I. Isolation of the Col Ib-P9 plasmid

R. E. Isaacson, J. Konisky

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The colicinogenic plasmid Col Ib-P9 of an Escherichia coli colicinogenic strain has been purified by agarose gel-filtration chromatography. The plasmid elutes from the column as a single peak, well separated from chromosomal fragments. When analyzed on neutral sucrose gradients the column, purified plasmid sediments at a rate characteristic for relaxed open circular Col Ib-P9 DNA. Measurements of the plasmid molecular complexity based on renaturation kinetics are consistent with the reported molecular weight for the plasmid. DNA-DNA hybridization has been employed to demonstrate a lack of sequence homology between the plasmid and the E. coli chromosome.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalMGG Molecular & General Genetics
Volume132
Issue number3
DOIs
StatePublished - Sep 1 1974

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