Studies on the O antigen of Salmonella typhosa. V. Enhancement of antibody response to protein antigens by the purified lipopolysaccharide

A. G. JOHNSON, S. GAINES, M. LANDY

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

Quantitative studies have demonstrated that a purified lipopolysaccharide (endotoxin) derived from the O-901 strain of Salmonella typhosa markedly enhanced the antibody response of rabbits when given separately or in conjunction with protein antigens. The augmentation of antibody levels varied from 2- to 40-fold with the number of injections, the dosage of antigen and endotoxin, and the route of administration. This antibody-enhancing property was found to be common to a broad group of endotoxins from Gram-negative bacilli and was not restricted to the lipopolysaccharide derived from S. typhosa. Factors affecting this enhancement were investigated, and data are presented which indicate that host susceptibility to endotoxin is a prerequisite for elevation of antibody levels; the intact lipopolysaccharide, as isolated, might not be essential for this activity; and the rate of clearance of antigen from the circulation of rabbits was accelerated when endotoxin was given in conjunction with protein. The data obtained are discussed in relation to postulated mechanisms on the antibody-enhancing action of endotoxin.

Original languageEnglish (US)
Pages (from-to)225-246
Number of pages22
JournalThe Journal of experimental medicine
Volume103
Issue number2
DOIs
StatePublished - Feb 1 1956

Fingerprint

O Antigens
Salmonella typhi
Endotoxins
Antibody Formation
Lipopolysaccharides
Antigens
Proteins
Blocking Antibodies
Rabbits
Antibodies
Bacillus
Injections

Keywords

  • ANTIGENS AND ANTIBODIES
  • POLYSACCHARIDES/effects
  • SALMONELLA TYPHOSA/metabolism

Cite this

@article{37d61c0d43104b9e855f3352ec4077fc,
title = "Studies on the O antigen of Salmonella typhosa. V. Enhancement of antibody response to protein antigens by the purified lipopolysaccharide",
abstract = "Quantitative studies have demonstrated that a purified lipopolysaccharide (endotoxin) derived from the O-901 strain of Salmonella typhosa markedly enhanced the antibody response of rabbits when given separately or in conjunction with protein antigens. The augmentation of antibody levels varied from 2- to 40-fold with the number of injections, the dosage of antigen and endotoxin, and the route of administration. This antibody-enhancing property was found to be common to a broad group of endotoxins from Gram-negative bacilli and was not restricted to the lipopolysaccharide derived from S. typhosa. Factors affecting this enhancement were investigated, and data are presented which indicate that host susceptibility to endotoxin is a prerequisite for elevation of antibody levels; the intact lipopolysaccharide, as isolated, might not be essential for this activity; and the rate of clearance of antigen from the circulation of rabbits was accelerated when endotoxin was given in conjunction with protein. The data obtained are discussed in relation to postulated mechanisms on the antibody-enhancing action of endotoxin.",
keywords = "ANTIGENS AND ANTIBODIES, POLYSACCHARIDES/effects, SALMONELLA TYPHOSA/metabolism",
author = "JOHNSON, {A. G.} and S. GAINES and M. LANDY",
year = "1956",
month = "2",
day = "1",
doi = "10.1084/jem.103.2.225",
language = "English (US)",
volume = "103",
pages = "225--246",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "2",

}

TY - JOUR

T1 - Studies on the O antigen of Salmonella typhosa. V. Enhancement of antibody response to protein antigens by the purified lipopolysaccharide

AU - JOHNSON, A. G.

AU - GAINES, S.

AU - LANDY, M.

PY - 1956/2/1

Y1 - 1956/2/1

N2 - Quantitative studies have demonstrated that a purified lipopolysaccharide (endotoxin) derived from the O-901 strain of Salmonella typhosa markedly enhanced the antibody response of rabbits when given separately or in conjunction with protein antigens. The augmentation of antibody levels varied from 2- to 40-fold with the number of injections, the dosage of antigen and endotoxin, and the route of administration. This antibody-enhancing property was found to be common to a broad group of endotoxins from Gram-negative bacilli and was not restricted to the lipopolysaccharide derived from S. typhosa. Factors affecting this enhancement were investigated, and data are presented which indicate that host susceptibility to endotoxin is a prerequisite for elevation of antibody levels; the intact lipopolysaccharide, as isolated, might not be essential for this activity; and the rate of clearance of antigen from the circulation of rabbits was accelerated when endotoxin was given in conjunction with protein. The data obtained are discussed in relation to postulated mechanisms on the antibody-enhancing action of endotoxin.

AB - Quantitative studies have demonstrated that a purified lipopolysaccharide (endotoxin) derived from the O-901 strain of Salmonella typhosa markedly enhanced the antibody response of rabbits when given separately or in conjunction with protein antigens. The augmentation of antibody levels varied from 2- to 40-fold with the number of injections, the dosage of antigen and endotoxin, and the route of administration. This antibody-enhancing property was found to be common to a broad group of endotoxins from Gram-negative bacilli and was not restricted to the lipopolysaccharide derived from S. typhosa. Factors affecting this enhancement were investigated, and data are presented which indicate that host susceptibility to endotoxin is a prerequisite for elevation of antibody levels; the intact lipopolysaccharide, as isolated, might not be essential for this activity; and the rate of clearance of antigen from the circulation of rabbits was accelerated when endotoxin was given in conjunction with protein. The data obtained are discussed in relation to postulated mechanisms on the antibody-enhancing action of endotoxin.

KW - ANTIGENS AND ANTIBODIES

KW - POLYSACCHARIDES/effects

KW - SALMONELLA TYPHOSA/metabolism

UR - http://www.scopus.com/inward/record.url?scp=0001295075&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001295075&partnerID=8YFLogxK

U2 - 10.1084/jem.103.2.225

DO - 10.1084/jem.103.2.225

M3 - Article

C2 - 13286429

AN - SCOPUS:0001295075

VL - 103

SP - 225

EP - 246

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 2

ER -