Studies on the O Antigen of Salmonella typhosa IV. Endotoxic Properties of the Purified Antigen

Maurice Landy, Arthur G. Johnson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A study was made of the endotoxic properties of a protein-free lipopolysac-charide isolated from the 0-901 strain of S. typhosa. The product was lethal for rabbits in a dose range of 20-50 μg while 300-500 μg were required to kill mice. The minimum pyrogenic dose in rabbits was 0.0002 μg, characterizing it as a pyrogen of extremely high potency. In a quantitative study of the local Shwartzman reaction, 1 μg or less, was effective as a preparatory or provocative dose. Its activity in eliciting bilateral renal cortical necrosis, the lesion characteristic of the generalized Shwartzman reaction, was in the range of 5-20 μg. Thus, very small quantities of a lipopolysaccharide, freed of protein, are capable of producing the various biological alterations generally attributed to endotoxins.Addendum. Since this report was submitted for publication preliminary tests of the effects of the typhosa lipopolysaccharide in man were undertaken(17). These indicate that when injected i.v. the minimum pyrogenic dose for a 70 kg subject lies in the range of.01-.04 μg.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume90
Issue number1
DOIs
StatePublished - Jan 1 1955

Fingerprint

Shwartzman Phenomenon
O Antigens
Salmonella typhi
Salmonella
Lipopolysaccharides
Kidney Cortex Necrosis
Rabbits
Pyrogens
Antigens
Endotoxins
Publications
Proteins

Cite this

@article{155e175a52f84c74940426134a646cf9,
title = "Studies on the O Antigen of Salmonella typhosa IV. Endotoxic Properties of the Purified Antigen",
abstract = "A study was made of the endotoxic properties of a protein-free lipopolysac-charide isolated from the 0-901 strain of S. typhosa. The product was lethal for rabbits in a dose range of 20-50 μg while 300-500 μg were required to kill mice. The minimum pyrogenic dose in rabbits was 0.0002 μg, characterizing it as a pyrogen of extremely high potency. In a quantitative study of the local Shwartzman reaction, 1 μg or less, was effective as a preparatory or provocative dose. Its activity in eliciting bilateral renal cortical necrosis, the lesion characteristic of the generalized Shwartzman reaction, was in the range of 5-20 μg. Thus, very small quantities of a lipopolysaccharide, freed of protein, are capable of producing the various biological alterations generally attributed to endotoxins.Addendum. Since this report was submitted for publication preliminary tests of the effects of the typhosa lipopolysaccharide in man were undertaken(17). These indicate that when injected i.v. the minimum pyrogenic dose for a 70 kg subject lies in the range of.01-.04 μg.",
author = "Maurice Landy and Johnson, {Arthur G.}",
year = "1955",
month = "1",
day = "1",
doi = "10.3181/00379727-90-21937",
language = "English (US)",
volume = "90",
pages = "57--62",
journal = "Experimental Biology and Medicine",
issn = "1535-3702",
publisher = "Sage Publications",
number = "1",

}

TY - JOUR

T1 - Studies on the O Antigen of Salmonella typhosa IV. Endotoxic Properties of the Purified Antigen

AU - Landy, Maurice

AU - Johnson, Arthur G.

PY - 1955/1/1

Y1 - 1955/1/1

N2 - A study was made of the endotoxic properties of a protein-free lipopolysac-charide isolated from the 0-901 strain of S. typhosa. The product was lethal for rabbits in a dose range of 20-50 μg while 300-500 μg were required to kill mice. The minimum pyrogenic dose in rabbits was 0.0002 μg, characterizing it as a pyrogen of extremely high potency. In a quantitative study of the local Shwartzman reaction, 1 μg or less, was effective as a preparatory or provocative dose. Its activity in eliciting bilateral renal cortical necrosis, the lesion characteristic of the generalized Shwartzman reaction, was in the range of 5-20 μg. Thus, very small quantities of a lipopolysaccharide, freed of protein, are capable of producing the various biological alterations generally attributed to endotoxins.Addendum. Since this report was submitted for publication preliminary tests of the effects of the typhosa lipopolysaccharide in man were undertaken(17). These indicate that when injected i.v. the minimum pyrogenic dose for a 70 kg subject lies in the range of.01-.04 μg.

AB - A study was made of the endotoxic properties of a protein-free lipopolysac-charide isolated from the 0-901 strain of S. typhosa. The product was lethal for rabbits in a dose range of 20-50 μg while 300-500 μg were required to kill mice. The minimum pyrogenic dose in rabbits was 0.0002 μg, characterizing it as a pyrogen of extremely high potency. In a quantitative study of the local Shwartzman reaction, 1 μg or less, was effective as a preparatory or provocative dose. Its activity in eliciting bilateral renal cortical necrosis, the lesion characteristic of the generalized Shwartzman reaction, was in the range of 5-20 μg. Thus, very small quantities of a lipopolysaccharide, freed of protein, are capable of producing the various biological alterations generally attributed to endotoxins.Addendum. Since this report was submitted for publication preliminary tests of the effects of the typhosa lipopolysaccharide in man were undertaken(17). These indicate that when injected i.v. the minimum pyrogenic dose for a 70 kg subject lies in the range of.01-.04 μg.

UR - http://www.scopus.com/inward/record.url?scp=0344686609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344686609&partnerID=8YFLogxK

U2 - 10.3181/00379727-90-21937

DO - 10.3181/00379727-90-21937

M3 - Article

VL - 90

SP - 57

EP - 62

JO - Experimental Biology and Medicine

JF - Experimental Biology and Medicine

SN - 1535-3702

IS - 1

ER -