Studies on nuclear binding of dehydroepiandrosterone in hepatocytes

Pamarthi F. Mohan, Margot P Cleary

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28 Scopus citations


Experiments were conducted to determine if dehydroepiandrosterone (DHEA) specifically binds to liver nuclei or interferes in the binding of other steroid hormones. Hepatocytes were isolated from obese, female Zucker rats that were treated with and without 0.6% DHEA in their diets for 2 weeks. The hepatocytes were incubated with either [3H]DHEA., [3H]estrone, or [3H]corticosterone. The nuclei isolated from the incubated cells from either control or DHEA-treated rats had no binding with [3H]DHEA, but had the expected binding with [3H]estrone and [3H] corticosterone. Furthermore, increasing concentrations of cold, unlabeled DHEA in the incubation media with [3H] estrone or [3H] corticosterone failed to have any effect on the binding of either of these steroid hormones to the nuclei. These results suggest that DHEA treatment does not exert its effects on cellular metabolism through a receptor-mediated mechanism like other steroid hormones or by interfering in the expression of steroid hormones such as estrone and corticosterone.

Original languageEnglish (US)
Pages (from-to)244-247
Number of pages4
Issue number5
StatePublished - May 1992

Bibliographical note

Funding Information:
GraduateS choolo f Medicine, MayoC linic( Rochester, MN, USA) for their help in settingu p the receptor assayp rocedureT. his work was supportedb y NIH ProgramP rojectG rantH L08214a ndt heH ormelF oun-dation.


  • Zucker rats
  • corticosterone
  • dehydroepiandrosterone
  • estrone
  • steroid
  • steroid binding hepatocyte


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