Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region

Ping Wang; Ruo Rong Cai; You Min Feng; You Shang Zhang

doi:10.1080/15216540050033203

Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region

Ping Wang, Ruo Rong Cai, You Min Feng, You Shang Zhang

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Single-chain insulin/IGF-1 hybrid-[Ins/IGF-1(C)], single-chain porcine insulin precursor-(PIP), and B10Asp PIP were prepared by protein engineering. Their growth-promoting activities in mouse breast cancer cell line GR2H6 are 10, 0.2, and 2 times that of insulin, respectively, and 29%, 0.6%, and 6% of that of IGF-1, indicating that the C domain and 9Glu of IGF-1 are important for its growth-promoting activity. Given these results and previous reports, we propose that the C domain, 9Glu, and 23Phe-26Asn beta bend are involved in the growth-promoting functional region of IGF-1.

Original language	English (US)
Pages (from-to)	321-325
Number of pages	5
Journal	IUBMB Life
Volume	49
Issue number	4
DOIs	https://doi.org/10.1080/15216540050033203
State	Published - 2000

Keywords

Growth-promoting functional region
Hybrid molecule
IGF-1
Insulin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1080/15216540050033203

Find It

Find It at U of M Libraries

Cite this

@article{ab746172621149b596a2b9decda55b98,

title = "Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region",

abstract = "Single-chain insulin/IGF-1 hybrid-[Ins/IGF-1(C)], single-chain porcine insulin precursor-(PIP), and B10Asp PIP were prepared by protein engineering. Their growth-promoting activities in mouse breast cancer cell line GR2H6 are 10, 0.2, and 2 times that of insulin, respectively, and 29\%, 0.6\%, and 6\% of that of IGF-1, indicating that the C domain and 9Glu of IGF-1 are important for its growth-promoting activity. Given these results and previous reports, we propose that the C domain, 9Glu, and 23Phe-26Asn beta bend are involved in the growth-promoting functional region of IGF-1.",

keywords = "Growth-promoting functional region, Hybrid molecule, IGF-1, Insulin",

author = "Ping Wang and Cai, \{Ruo Rong\} and Feng, \{You Min\} and Zhang, \{You Shang\}",

year = "2000",

doi = "10.1080/15216540050033203",

language = "English (US)",

volume = "49",

pages = "321--325",

journal = "IUBMB Life",

issn = "1521-6543",

publisher = "John Wiley and Sons Inc.",

number = "4",

}

TY - JOUR

T1 - Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region

AU - Wang, Ping

AU - Cai, Ruo Rong

AU - Feng, You Min

AU - Zhang, You Shang

PY - 2000

Y1 - 2000

N2 - Single-chain insulin/IGF-1 hybrid-[Ins/IGF-1(C)], single-chain porcine insulin precursor-(PIP), and B10Asp PIP were prepared by protein engineering. Their growth-promoting activities in mouse breast cancer cell line GR2H6 are 10, 0.2, and 2 times that of insulin, respectively, and 29%, 0.6%, and 6% of that of IGF-1, indicating that the C domain and 9Glu of IGF-1 are important for its growth-promoting activity. Given these results and previous reports, we propose that the C domain, 9Glu, and 23Phe-26Asn beta bend are involved in the growth-promoting functional region of IGF-1.

AB - Single-chain insulin/IGF-1 hybrid-[Ins/IGF-1(C)], single-chain porcine insulin precursor-(PIP), and B10Asp PIP were prepared by protein engineering. Their growth-promoting activities in mouse breast cancer cell line GR2H6 are 10, 0.2, and 2 times that of insulin, respectively, and 29%, 0.6%, and 6% of that of IGF-1, indicating that the C domain and 9Glu of IGF-1 are important for its growth-promoting activity. Given these results and previous reports, we propose that the C domain, 9Glu, and 23Phe-26Asn beta bend are involved in the growth-promoting functional region of IGF-1.

KW - Growth-promoting functional region

KW - Hybrid molecule

KW - IGF-1

KW - Insulin

UR - https://www.scopus.com/pages/publications/0033946267

UR - https://www.scopus.com/inward/citedby.url?scp=0033946267&partnerID=8YFLogxK

U2 - 10.1080/15216540050033203

DO - 10.1080/15216540050033203

M3 - Article

C2 - 10995036

AN - SCOPUS:0033946267

SN - 1521-6543

VL - 49

SP - 321

EP - 325

JO - IUBMB Life

JF - IUBMB Life

IS - 4

ER -

Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region

Abstract

Keywords

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this