Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region

Ping Wang, Ruo Rong Cai, You Min Feng, You Shang Zhang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Single-chain insulin/IGF-1 hybrid-[Ins/IGF-1(C)], single-chain porcine insulin precursor-(PIP), and B10Asp PIP were prepared by protein engineering. Their growth-promoting activities in mouse breast cancer cell line GR2H6 are 10, 0.2, and 2 times that of insulin, respectively, and 29%, 0.6%, and 6% of that of IGF-1, indicating that the C domain and 9Glu of IGF-1 are important for its growth-promoting activity. Given these results and previous reports, we propose that the C domain, 9Glu, and 23Phe-26Asn beta bend are involved in the growth-promoting functional region of IGF-1.

Original languageEnglish (US)
Pages (from-to)321-325
Number of pages5
JournalIUBMB Life
Volume49
Issue number4
StatePublished - Jul 12 2000

Fingerprint

Insulin-Like Growth Factor I
Insulin
Growth
Insulin, Regular, Pork
Protein Engineering
Cells
Breast Neoplasms
Cell Line
Proteins
growth promoting activity

Keywords

  • Growth-promoting functional region
  • Hybrid molecule
  • IGF-1
  • Insulin

Cite this

Wang, P., Cai, R. R., Feng, Y. M., & Zhang, Y. S. (2000). Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region. IUBMB Life, 49(4), 321-325.

Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region. / Wang, Ping; Cai, Ruo Rong; Feng, You Min; Zhang, You Shang.

In: IUBMB Life, Vol. 49, No. 4, 12.07.2000, p. 321-325.

Research output: Contribution to journalArticle

Wang, P, Cai, RR, Feng, YM & Zhang, YS 2000, 'Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region', IUBMB Life, vol. 49, no. 4, pp. 321-325.
Wang, Ping ; Cai, Ruo Rong ; Feng, You Min ; Zhang, You Shang. / Studies on insulin/IGF-1 hybrid and IGF-1 growth-promoting functional region. In: IUBMB Life. 2000 ; Vol. 49, No. 4. pp. 321-325.
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