TY - JOUR
T1 - Studies on a Nigerian isolate of banana streak badnavirus
T2 - I. Purification and enzyme-linked immunosorbent assay
AU - Thottappilly, G.
AU - Dahal, G.
AU - Lockhart, B. E L
PY - 1998/4
Y1 - 1998/4
N2 - A Nigerian isolate of banana streak badnavirus (BSV) was purified and a polyclonal antiserum was produced in mice. The antiserum titre was between 1:10 000 and 1:40 000 in enzyme-linked immunosorbent assay (ELISA), and showed a good specificity to BSV antigens. Comparative tests were carded out to determine the sensitivity and reliability of BSV antigen detection by double antibody sandwich (DAS)-ELISA, triple antibody sandwich (TAS)-ELISA, antigen coated plate (ACP)-ELISA, and protein-A coated antibody sandwich (PAS)-ELISA. TAS-ELISA using rabbit polyclonal antiserum to trap BSV and mouse polyclonal antiserum to detect the virus particles, was more sensitive than ACP-ELISA and PAS-ELISA and detected BSV in plant extracts from both symptomatic and some asymptomatic plants. However, immunosorbent electron microscopy detected more BSV-infected plants from asymptomatic plant samples than did TAS-ELISA. Results of this study showed that detection of BSV antigens in sap extracts by TAS-ELISA was most efficient with symptomatic tissues which occurred most frequently in the 'cool rainy' season. This suggests that for more reliable BSV-indexing of field samples, tissue sampling should be done during the rainy season when most BSV-infected plants express severe symptoms.
AB - A Nigerian isolate of banana streak badnavirus (BSV) was purified and a polyclonal antiserum was produced in mice. The antiserum titre was between 1:10 000 and 1:40 000 in enzyme-linked immunosorbent assay (ELISA), and showed a good specificity to BSV antigens. Comparative tests were carded out to determine the sensitivity and reliability of BSV antigen detection by double antibody sandwich (DAS)-ELISA, triple antibody sandwich (TAS)-ELISA, antigen coated plate (ACP)-ELISA, and protein-A coated antibody sandwich (PAS)-ELISA. TAS-ELISA using rabbit polyclonal antiserum to trap BSV and mouse polyclonal antiserum to detect the virus particles, was more sensitive than ACP-ELISA and PAS-ELISA and detected BSV in plant extracts from both symptomatic and some asymptomatic plants. However, immunosorbent electron microscopy detected more BSV-infected plants from asymptomatic plant samples than did TAS-ELISA. Results of this study showed that detection of BSV antigens in sap extracts by TAS-ELISA was most efficient with symptomatic tissues which occurred most frequently in the 'cool rainy' season. This suggests that for more reliable BSV-indexing of field samples, tissue sampling should be done during the rainy season when most BSV-infected plants express severe symptoms.
KW - Banana streak badnavirus
KW - Immunosorbent electron microscopy
KW - Polyclonal antibody
KW - TAS-ELISA
KW - Virus detection
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U2 - 10.1111/j.1744-7348.1998.tb05201.x
DO - 10.1111/j.1744-7348.1998.tb05201.x
M3 - Article
AN - SCOPUS:0031806316
SN - 0003-4746
VL - 132
SP - 253
EP - 261
JO - Annals of Applied Biology
JF - Annals of Applied Biology
IS - 2
ER -