Studies of vegetative compatibility-incompatibility in higher plants - VI. Grafting of Sedum and Solanum callus tissue in vitro

R. Moore, D. B. Walker

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In order to determine whether graft incompatibility between Sedum telephoides and Solanum pennellii required a prior wounding of tissues, we monitored the cellular events that occurred during grafting of intact (i.e., non-wounded) surfaces of Sedum and Solanum callus tissue in vitro. The only cytological events that occurred in response to autografting of Sedum callus in vitro were (1) dilation of the endoplasmic reticulum in cells adjacent to the graft interface, and (2) the formation of a conspicuously thickened cell wall between the grafted cells. The only cytological event that occurred in callus cells of Solanum that were heterografted to callus cells of Sedum was a slightly reduced staining intensity of the cytoplasm. Sedum cells of the heterograft, on the other hand, underwent a progressive cellular necrosis when grafted to Solanum in vitro. These results indicate that (1) a prior wounding of tissues is not necessary to elicit the incompatibility response between Sedum and Solanum, although the wounding induced from an initial graft incision acts to exaggerate and accelerate incompatibility, and (2) graft incompatibility between Sedum and Solanum occurs in vitro between callus cells as it does in vivo between stems. Thus, the incompatibility factor is probably a normal component of the ground tissue and not the exclusive product of more highly differentiated tissues. Finally, the mechanism of autografting of non-wounded callus cells is compared to the known mechanism of postgenital tissue fusions.

Original languageEnglish (US)
Pages (from-to)114-121
Number of pages8
JournalProtoplasma
Volume115
Issue number2-3
DOIs
StatePublished - Jun 1 1983

    Fingerprint

Keywords

  • Compatibility
  • Grafting
  • Incompatibility
  • Sedum telephoides
  • Solanum pennellii
  • Tissue culture

Cite this