Studies of the metabolism of separated bone cells - I. Techniques of separation and identification

D. M. Smith, C. C. Johnston, A. R. Severson

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The mechanical separation of rat skeletal tissue into viable populations of periosteal cells, osteoblasts, osteocytes, and marrow cells has been undertaken. Periosteal cells and osteocytes were obtained as tissue preparations, while osteoblasts and marrow cells were isolated as individual cells. The cell populations were identified during the preparative procedure by their histological and histochemical appearance. Viability of these preparations was demonstrated by their metabolic activity during short-term incubations and their histological appearance and vital staining before and after such incubations. The cell populations produced carbon dioxide and lactate, consumed oxygen, and incorporated uridine into RNA. Comparison of uridine incorporation into RNA of cells from control and parathyroid hormone treated rats was compatible with a differential effect of parathyroid hormone on the cell populations. These findings indicate that the technique described can be utilized to produce viable bone cells of different types, and may offer a new means for characterizing the role of each cell type in the metabolism of skeletal tissue.

Original languageEnglish (US)
Pages (from-to)56-69
Number of pages14
JournalCalcified Tissue Research
Volume11
Issue number1
DOIs
StatePublished - Jan 1 1973

Fingerprint

Bone and Bones
Osteocytes
Uridine
Parathyroid Hormone
Osteoblasts
Population
Bone Marrow
RNA
Carbon Dioxide
Lactic Acid
Staining and Labeling
Oxygen

Keywords

  • Bone
  • Cells
  • Marrow
  • Osteoblasts
  • Osteocytes
  • Periosteum

Cite this

Studies of the metabolism of separated bone cells - I. Techniques of separation and identification. / Smith, D. M.; Johnston, C. C.; Severson, A. R.

In: Calcified Tissue Research, Vol. 11, No. 1, 01.01.1973, p. 56-69.

Research output: Contribution to journalArticle

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