Studies of streptococcal membrane antigen-binding cells in acute rheumatic fever

R. C. Williams, V. Raizada, K. Prakash, K. B. Sharma, Inder Anand, N. K. Ganguly, J. B. Zabriskie

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Abstract

Blood specimens from patients with rheumatic heart disease in both India and New Mexico were typed for the presence of B cell alloantigen 883 by use of a mouse monoclonal antibody with identical specificity to the original 883 human alloantiserum. Strong relative segregation was recorded for 883 positive B cell typing in patients with rheumatic heart disease in both geographic locations as compared with that in normal unaffected controls. In patients with acute rheumatic fever, studies of actual B-lymphocyte membrane binding by anti-883 monoclonal antibody and sonicated group A streptococcal membrane antigens showed separate but contiguous localization on isolated cell surfaces. Although physically distinct, 883 B cell alloantigen and sonicated group A streptococcal membrane antigens moved together in cell capping studies after incubation at 37°C. These findings reaffirm the apparent close association between 883 B cell alloantigen and rheumatic heart disease. They also demonstrate that the B cell alloantigen 883 itself is physically distinct from but very close to sites on antigen-reactive B cells actually binding to group A streptococcal membrane antigens.

Original languageEnglish (US)
Pages (from-to)531-536
Number of pages6
JournalJournal of Laboratory and Clinical Medicine
Volume105
Issue number5
StatePublished - Oct 31 1985

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    Williams, R. C., Raizada, V., Prakash, K., Sharma, K. B., Anand, I., Ganguly, N. K., & Zabriskie, J. B. (1985). Studies of streptococcal membrane antigen-binding cells in acute rheumatic fever. Journal of Laboratory and Clinical Medicine, 105(5), 531-536.