Studies of lysosomal function: I. Metabolism of some complex lipids by isolated hepatocytes and Kupffer cells

John A. Barranger; Peter G. Pentchev; F. Scott Furbish; Clifford J. Steer; E. Anthony Jones; Roscoe O. Brady

doi:10.1016/0006-291X(78)91502-4

Studies of lysosomal function: I. Metabolism of some complex lipids by isolated hepatocytes and Kupffer cells

John A. Barranger
, Peter G. Pentchev
, F. Scott Furbish
, Clifford J. Steer
, E. Anthony Jones
, Roscoe O. Brady

Administration (TMED)

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The enzymatic hydrolysis of four complex lipids was measured in extracts of rat hepatocytes and Kupffer cells. Sphingomyelin, glucocerebroside, ceramide trihexoside, and G_Ml-ganglioside were hydrolyzed by extracts of both cell types. Hepatocytes contain 90% or more of the complex lipid hydrolases present in liver. The activities of ten additional acid hydrolases are located predominately in hepatocytes with only a small fraction of the total activity present in Kupffer cells.

Original language	English (US)
Pages (from-to)	1055-1060
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	83
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(78)91502-4
State	Published - Aug 14 1978

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@article{6ea9f68a0858457e99ca647e952e8d0d,

title = "Studies of lysosomal function: I. Metabolism of some complex lipids by isolated hepatocytes and Kupffer cells",

abstract = "The enzymatic hydrolysis of four complex lipids was measured in extracts of rat hepatocytes and Kupffer cells. Sphingomyelin, glucocerebroside, ceramide trihexoside, and GMl-ganglioside were hydrolyzed by extracts of both cell types. Hepatocytes contain 90\% or more of the complex lipid hydrolases present in liver. The activities of ten additional acid hydrolases are located predominately in hepatocytes with only a small fraction of the total activity present in Kupffer cells.",

author = "Barranger, \{John A.\} and Pentchev, \{Peter G.\} and \{Scott Furbish\}, F. and Steer, \{Clifford J.\} and \{Anthony Jones\}, E. and Brady, \{Roscoe O.\}",

year = "1978",

month = aug,

day = "14",

doi = "10.1016/0006-291X(78)91502-4",

language = "English (US)",

volume = "83",

pages = "1055--1060",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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TY - JOUR

T1 - Studies of lysosomal function

T2 - I. Metabolism of some complex lipids by isolated hepatocytes and Kupffer cells

AU - Barranger, John A.

AU - Pentchev, Peter G.

AU - Scott Furbish, F.

AU - Steer, Clifford J.

AU - Anthony Jones, E.

AU - Brady, Roscoe O.

PY - 1978/8/14

Y1 - 1978/8/14

N2 - The enzymatic hydrolysis of four complex lipids was measured in extracts of rat hepatocytes and Kupffer cells. Sphingomyelin, glucocerebroside, ceramide trihexoside, and GMl-ganglioside were hydrolyzed by extracts of both cell types. Hepatocytes contain 90% or more of the complex lipid hydrolases present in liver. The activities of ten additional acid hydrolases are located predominately in hepatocytes with only a small fraction of the total activity present in Kupffer cells.

AB - The enzymatic hydrolysis of four complex lipids was measured in extracts of rat hepatocytes and Kupffer cells. Sphingomyelin, glucocerebroside, ceramide trihexoside, and GMl-ganglioside were hydrolyzed by extracts of both cell types. Hepatocytes contain 90% or more of the complex lipid hydrolases present in liver. The activities of ten additional acid hydrolases are located predominately in hepatocytes with only a small fraction of the total activity present in Kupffer cells.

UR - https://www.scopus.com/pages/publications/0017893996

UR - https://www.scopus.com/pages/publications/0017893996#tab=citedBy

U2 - 10.1016/0006-291X(78)91502-4

DO - 10.1016/0006-291X(78)91502-4

M3 - Article

C2 - 708424

AN - SCOPUS:0017893996

SN - 0006-291X

VL - 83

SP - 1055

EP - 1060

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Studies of lysosomal function: I. Metabolism of some complex lipids by isolated hepatocytes and Kupffer cells

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