TY - JOUR
T1 - Studies of feedback suppression of bile salt synthesis in the bile-fistula rat
AU - Duane, William C
AU - McHale, A. P.
AU - Hamilton, J. N.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - We have previously reported that intravenous infusion of taurocholate at 10 μmol/(100 g. hr) into bile-fistula rats suppressed bile salt synthesis by 85% (Pries et al. 1983. J. Lipid Res. 24: 141-146). Recently, however, infusion rates twice this high have been reported not to suppress synthesis (Davis et al. 1984. Falk Symposium 42. MTP Press Ltd., Boston, 37-45). Because the only major difference in design of these two studies was supplementation with sodium bicarbonate to replace biliary losses induced by bile salt choleresis, we have repeated our studies with and without bicarbonate supplementation. Without bicarbonate, as before, we found suppression of synthesis during infusion of taurocholate at 10 μmol/(100 g·hr). With bicarbonate, no suppression of synthesis occurred at these infusion rates. These data indicate that bicarbonate supplementation is essential when testing physiological effects of infused bile salt in the bile-fistula rat.
AB - We have previously reported that intravenous infusion of taurocholate at 10 μmol/(100 g. hr) into bile-fistula rats suppressed bile salt synthesis by 85% (Pries et al. 1983. J. Lipid Res. 24: 141-146). Recently, however, infusion rates twice this high have been reported not to suppress synthesis (Davis et al. 1984. Falk Symposium 42. MTP Press Ltd., Boston, 37-45). Because the only major difference in design of these two studies was supplementation with sodium bicarbonate to replace biliary losses induced by bile salt choleresis, we have repeated our studies with and without bicarbonate supplementation. Without bicarbonate, as before, we found suppression of synthesis during infusion of taurocholate at 10 μmol/(100 g·hr). With bicarbonate, no suppression of synthesis occurred at these infusion rates. These data indicate that bicarbonate supplementation is essential when testing physiological effects of infused bile salt in the bile-fistula rat.
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M3 - Article
C2 - 2835417
AN - SCOPUS:0023870692
VL - 29
SP - 212
EP - 214
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 2
ER -