Structures of rhodopsin kinase in different ligand states reveal key elements involved in G protein-coupled receptor kinase activation

Puja Singh, Benlian Wang, Tadao Maeda, Krzysztof Palczewski, John J.G. Tesmer

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

G protein-coupled receptor (GPCR) kinases (GRKs) phosphorylate activated heptahelical receptors, leading to their uncoupling from G proteins. Here we report six crystal structures of rhodopsin kinase (GRK1), revealing not only three distinct nucleotide-binding states of a GRK but also two key structural elements believed to be involved in the recognition of activated GPCRs. The first is the C-terminal extension of the kinase domain, which was observed in all nucleotide-bound GRK1 structures. The second is residues 5-30 of the N terminus, observed in one of the GRK1·(Mg2+) 2·ATP structures. The N terminus was also clearly phosphorylated, leading to the identification of two novel phosphorylation sites by mass spectral analysis. Co-localization of the N terminus and the C-terminal extension near the hinge of the kinase domain suggests that activated GPCRs stimulate kinase activity by binding to this region to facilitate full closure of the kinase domain.

Original languageEnglish (US)
Pages (from-to)14053-14062
Number of pages10
JournalJournal of Biological Chemistry
Volume283
Issue number20
DOIs
StatePublished - May 16 2008

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