Structure of the murine Jak2 protein-tyrosine kinase and its role in interleukin 3 signal transduction

Olli Silvennoinen, Bruce A. Witthuhn, Frederick W. Quelle, John L. Cleveland, Taolin Yi, James N. Ihle

Research output: Contribution to journalArticlepeer-review

424 Scopus citations

Abstract

Interleukin 3 (IL-3) regulates the proliferation and differentiation of hematopoietic cells. Although the IL-3 receptor chains lack kinase catalytic domains, IL-3 induces tyrosine phosphorylation of cellular proteins. To investigate the potential role of the JAK family of protein-tyrosine kinases in IL-3 signal transduction, we have obtained full-length cDNA clones for murine Jak1 and Jak2 protein-tyrosine kinases and prepared antiserum against the predicted proteins. Using antisera against Jak2, we demonstrate that IL-3 stimulation results in the rapid and specific tyrosine phosphorylation of Jak2 and activates its in vitro kinase activity.

Original languageEnglish (US)
Pages (from-to)8429-8433
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number18
DOIs
StatePublished - Sep 15 1993

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