Structure-Function Guided Extraction and Scale-Up of Pea Protein Isolate Production

Lucy Hansen, Fan Bu, Baraem P. Ismail

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The lack of adequate guidance and control of the extraction conditions as well as the gap between bench- and industrial-scale production, contributes to the poor functionality of commercial pea protein isolate (cPPI). Therefore, pea protein extraction conditions were evaluated and scaled up to maximize protein purity and yield, while maintaining structural integrity, following mild alkaline solubilization with isoelectric precipitation and salt solubilization coupled with membrane filtration. Both extraction methods resulted in high protein yield (>64%) and purity (>87%). Structure-function characterization illustrated the preserved structural integrity of PPI samples and their superior solubility, gelation, and emulsification properties compared to cPPI. Results confirmed, for the first time, that double solubilization at mild pH (7.5) can replace single solubilization at high alkalinity and achieve a similar yield while preserving structural integrity. Additionally, this study demonstrated, the scalability of the benchtop salt extraction coupled with ultrafiltration/diafiltration. Scaling up the production eliminated some structural and functional differences between the salt-extracted PPI and pH-extracted PPI. Scaling-up under mild and controlled conditions resulted in partial denaturation and a low degree of polymerization, coupled with the superior functionality of the produced isolates compared to cPPI. Results of this work can be used as a benchmark to guide the industrial production of functional pea protein ingredients.

Original languageEnglish (US)
Article number3773
JournalFoods
Volume11
Issue number23
DOIs
StatePublished - Dec 2022

Bibliographical note

Funding Information:
This work was supported by funds from the United States Department of Agriculture/Agriculture Research Station—Pulse Crop Health Initiative (Agreement number 58-3060-8-024).

Publisher Copyright:
© 2022 by the authors.

Keywords

  • benchtop versus pilot plant production
  • pH extraction
  • pea protein isolate
  • protein structure and functionality
  • salt extraction
  • ultrafiltration

PubMed: MeSH publication types

  • Journal Article

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