The Actinobacillus actinomycetemcomitans leukotoxin gene complex, consisting of four genes, has been cloned and the sequence of the AaLtC and AaLtA genes reported. The present paper details the sequences of the AaLtB and AaLtD genes which, like AaLtC and AaLtA, are also homologues of genes found in other cytolytic toxin complexes of several other Gram-negative bacterial pathogens. When tested in a recombinant expression system, the AaLtB and/or AaLtD genes are required for the translocation and insertion of the A. actinomycetemcomitans leukotoxin (AaLtA) into the cell membrane of Escherichia coli.
|Original language||English (US)|
|Number of pages||11|
|State||Published - Aug 1991|
Bibliographical noteFunding Information:
This work was supported by National Institutes of Health grants DE07118, DE08239 and D E09517 .
- Actinobacillus actinomycetemcomitans
- bacterial toxin
- immunohistochemical localization
- molecular cloning
- transport protein
- virulence factor